中国瓜菜2025，Vol.38Issue(4)：67-72,6.DOI:10.16861/j.cnki.zggc.2024.0314
文成糯米山药染色体核型分析及基因组大小预估
Chromosome karyotype analysis and genome size estimation of Wencheng glutinous rice yam
摘要
Abstract
Flow cytometry was used to analyze the number and morphology of chromosomes in Wencheng glutinous rice yam,while flow cytometry was employed to identify the chromosomes karyotype and estimate its genome size.Using young stem segments of Wencheng glutinous rice yam as experimental materials,flame drying method was employed to obtain mitotic metaphase chromosome sections.DAPI staining fluorescence observation was used to count the chromo-somes,and then the karyotype was analyzed.Diploid shield leaf yam tender leaves and com(B73)were used as internal references,and flow cytometry was applied to determine the genome size and ploidy of Wencheng glutinous rice yam.The results showed that the number of chromosomes in Wencheng glutinous rice yam was 40,with a karyotype formula of 2n=2x=40=40 m,classified as lB type,representing a relatively primitive evolutionary type.The genome size of Wencheng glutinous rice yam was estimated to be 0.59 Gb.This results provide a basis for subsequent genetic improve-ment and breeding.关键词
文成糯米山药/倍性鉴定/核型分析/基因组大小Key words
Wencheng glutinous rice yam/Ploidy identification/Karyotype analysis/Genome size分类
农业科技引用本文复制引用
许竹溦,邓逸桐,雷俊,邵晓伟,姜欢,余文慧..文成糯米山药染色体核型分析及基因组大小预估[J].中国瓜菜,2025,38(4):67-72,6.基金项目
非粮化耕地旱作粮油高产高效关键技术集成与示范(2023SJLM17) （2023SJLM17）
衢州市科技项目(2024K065) （2024K065）
浙江省农业新品种选育重大科技专项(2021C02064-1) （2021C02064-1）
江苏省根茎类种质资源库项目 （）
