中国农业科学2025，Vol.58Issue(8)：1463-1478,16.DOI:10.3864/j.issn.0578-1752.2025.08.001
OsDREB1J调控水稻籽粒大小的功能研究
The Function of OsDREB1J in Regulating Rice Grain Size
摘要
Abstract
[Objective]The AP2/ERF(APETALA2/ethylene responsive factor)superfamily is a group of transcription factors that play important regulatory roles in plant growth and development,as well as in response to adverse environmental stressors.The AP2/ERF transcription factors are widely present and have many members in plants.Exploring the function of AP2/ERF family gene on grain size provides important genetic resources for regulating grain shape in rice.[Method]OsDREB1J gene(LOC_Os08g43200)was cloned by homologous recombination,and its basic characteristics,tissue expression characteristics,and the relative expression patterns under plant hormones were analyzed by bioinformatics and qRT-PCR.The transactivation activity and subcellular localization of OsDREB1J were analyzed by yeast heterologous expression,transient expression of rice protoplasts and tobacco.The overexpression and knockout mutant transgenic rice plants of OsDREB1J were obtained by genetic transformation system,and the grain size phenotypes were analyzed by phenotypic analysis technology.[Result]Subcellular localization analysis showed that OsDREB1J was localized in the nucleus.Bioinformatics showed that the full-length coding sequence of OsDREB1J was 711 bp,encoding 236 amino acids.OsDREB1J protein had no transmembrane structure,and the molecular weight of 27.47 kDa,the theoretical isoelectric point of 5.54,and had a conserved AP2 domain unique to the AP2/ERF family.The cis-acting elements analysis of OsDREB1J promoter showed that the promoter contained cis-acting elements related to hormone response,light and stresses response.The qRT-PCR analysis showed that OsDREB1J was expressed in different tissues of rice with no tissue specificity,and the relative expression level in panicle was the highest.At the same time,OsDREB1J was induced or reduced by different hormone.Transcriptional activation analysis showed that the full-length of OsDREB1J has no transcriptional activity,but the C-terminal fragment was sufficient for the transactivation ability.Phenotypic analysis showed that the grain length,length-width ratio and thousand grain weight of osdreb1j mutant were significantly higher than those of ZH11,OsDREB1J overexpression transgenic rice plants displayed opposite phenotypes,while changing the expression of OsDREB1J did not affect rice grain width.These results show that OsDREB1J may affect grain size by regulating cell length rather than cell proliferation and cell expansion.[Conclusion]In conclusion,OsDREB1J may be involved in regulating rice grain size through hormone signaling pathway.关键词
水稻/OsDREB1J/转录因子/籽粒大小/亚细胞定位/表达模式Key words
rice/OsDREB1J/transcription factor/grain size/subcellular localization/expression pattern引用本文复制引用
韦萍,潘炬忠,朱德平,邵胜雪,陈珊珊,韦雅倩,高维维..OsDREB1J调控水稻籽粒大小的功能研究[J].中国农业科学,2025,58(8):1463-1478,16.基金项目
国家自然科学基金青年基金(32301749)、广西自然科学基金青年基金(2024GXNSFBA010324) （32301749）
