中国中医急症2025，Vol.34Issue(4)：587-592,6.DOI:10.3969/j.issn.1004-745X.2025.04.005
基于PINK1/Parkin通路探寻心复康口服液改善H9c2心肌细胞肥大的作用机制
Exploring on Mechanism of Xinfukang Oral Liquid in Improving H9c2 Cardiomyocyte Hypertrophy Based on the PINK1/Parkin Pathway
摘要
Abstract
Objective:To explore the effect and mechanism of Xinfukang oral liquid on hypertrophy of H9c2 cardiomyocytes based on the PTEN-induced putative kinase 1(PINK1)/E3 ubiquitin ligase(Parkin)pathway.Methods:A hypertrophy model was established by treating H9c2 cells with 20 μmol/L phenylephrine(PE).Suc-cessfully modeled cells were then treated with optimal concentrations of Xinfukang-contained serum(Xinfukang group)or blank serum(model group),with an additional normal control group.The optimal concentration was de-termined using the CCK-8 assay.Mitochondrial morphology,Tom20 expression,mitochondrial membrane potential,reactive oxygen species(ROS)content,mitochondrial respiratory function,and adenosine triphosphate(ATP)levels were measured.Protein expressions of PINK 1,Parkin,voltage-dependent anion channel 1(VDAC1),inositol 1,4,5-trisphosphate receptor(IP3R),and glucose-regulated protein 75(Grp75)were detected.mRNA expressions of B-type natriuretic peptide(BNP),atrial natriuretic peptide(ANP),PINK 1,Parkin,caspase-3,and caspase-9 were al-so measured.Results:Compared with the control group,the model group showed increased BNP and ANP expres-sion,mitochondrial length,and ROS expression(P＜0.05),decreased Tom20,mitochondrial membrane potential,mi-tochondrial respiratory function,and ATP synthesis levels(P＜0.01),reduced PINK1 and Parkin protein and mRNA expression(P＜0.01),and elevated VDAC1,IP3R,Grp75 protein expression,and caspase-3/-9 mRNA ex-pression(P＜0.05).Compared with the model group,the Xinfukang group exhibited decreased mitochondrial length and ROS expression(P＜0.05),increased Tom20,mitochondrial membrane potential,respiratory function,and ATP levels(P＜0.05),enhanced Parkin protein and mRNA expression(P＜0.01),and reduced VDAC1,IP3R,Grp75 protein expression,and caspase-3/-9 mRNA expression(P＜0.05).Conclusion:Xinfukang oral liquid ame-liorates mitochondrial morphology and functions in hypertrophic H9c2 cardiomyocytes and inhibits cardiomyocyte apoptosis by activating the PINK1/Parkin mitophagy pathway.关键词
H9c2心肌细胞/心复康口服液/PINK1/Parkin通路/线粒体功能/细胞凋亡Key words
H9c2 cardiomyocyte/Xinfukang oral liquid/PINK1/Parkin pathway/Mitochondrial function/Apoptosis分类
医药卫生引用本文复制引用
李家然,张笑涵,邱志凌,宋宇国,胡元会,杜柏..基于PINK1/Parkin通路探寻心复康口服液改善H9c2心肌细胞肥大的作用机制[J].中国中医急症,2025,34(4):587-592,6.基金项目
国家自然科学基金资助项目(82074409,82205097) （82074409,82205097）
中国中医科学院广安门医院引航工程临床类项目(HLCMHPP2023106) （HLCMHPP2023106）
