中国农业科学2025，Vol.58Issue(8)：1579-1590,12.DOI:10.3864/j.issn.0578-1752.2025.08.009
长期不同施肥对棕壤细菌群落结构和碳代谢功能的影响
Eeffects of Long-Term Fertilization on Bacterial Community Structure and Carbon Metabolic Functions in Brown Soil
摘要
Abstract
[Objective]This study aimed to elucidate the relationships among bacterial population structure,key species,carbon metabolic functions,and variations in soil physicochemical and biological properties resulting from long-term different fertilization treatments in agricultural soils.[Method]The amplicon sequencing technology based on the molecular marker of bacterial 16S rRNA were employed to analyze soil bacterial community structure,ecological networks,potential carbon metabolic functions,and their correlations with soil physicochemical and biological properties after 29 years of continuous application of various fertilization treatments(no fertilization as control,CK;chemical fertilizers,N4;and reduced application of chemical fertilizer combined with organic manure,M2N2)at the Long-term Positioning Experimental Station at Shenyang Agricultural University.[Result]Different fertilization treatments significantly altered soil physicochemical and biological properties,and bacterial populations,diversity,and abundance of potential carbon metabolic genes.Compared with CK treatment,N4 treatment significantly decreased soil pH value,bacterial abundance and community diversity,which indicated that M2N2 treatment demonstrated a beneficial maintenance effect.Although long-term fertilization practices(both N4 and M2N2)significantly increased soil respiration rates,they also markedly reduced net nitrogen(N)mineralization rates at 0-20 cm soil layer.Furthermore,compared with N4 treatment,M2N2 treatment significantly enhanced soil net N mineralization rates.Soil ammonium N content,net N mineralization rate and pH value were the critical environmental factors influencing soil bacterial populations.Network co-occurrence analysis revealed that Bradyrhizobium elkanii and beta proteobacterium WWH154 were the key bacterial species that maintained the stability of bacterial ecological networks,and about 100 dominant bacterial species co-occurred fully with beta proteobacterium WWH154 and 54%of the species co-occurred with Bradyrhizobium elkanii.Long-term fertilization(N4 and M2N2)increased the relative abundance of two key species by 61.9%-169.4%,especially the M2N2 treatment.The function prediction of carbon metabolic genes showed that N4 treatment reduced the abundance of various carbon metabolism-related genes,such as carbon fixation pathways in prokaryotes,Aminoacyl tRNA biosynthesis and Amino acid related enzymes in soil bacteria,and M2N2 treatment significantly stabilized the carbon metabolic pathways.[Conclusion]In summary,long-term fertilization altered the physicochemical and biological properties of agricultural soil.Reduced application of chemical fertilizer combined with organic manure enriched key bacterial species and increased the complexity of microbial networks,which would be beneficial to coping with environmental changes,thus maintaining soil ecological functions and increasing crop yield.关键词
长期施肥/棕壤/关键物种/细菌多样性/网络共现Key words
long-term fertilization/brown soil/key bacterial species/bacterial diversity/network co-occurrence引用本文复制引用
白雨欣,刘灵芝,安婷婷,李双异,汪景宽..长期不同施肥对棕壤细菌群落结构和碳代谢功能的影响[J].中国农业科学,2025,58(8):1579-1590,12.基金项目
国家重点研发计划(2021YFD1500205)、辽宁省高等学校基本科研项目(LJKMZ20220995) （2021YFD1500205）
