中国中医急症2025，Vol.34Issue(4)：593-597,5.DOI:10.3969/j.issn.1004-745X.2025.04.006
芪参益气滴丸对糖氧剥夺/复氧H9c2心肌细胞的保护作用及机制研究
Protective Effect and Mechanism of Qishen Yiqi Dropping Pills on Glyco-oxygen-deprived/reoxygenated H9c2 Cardiomyocytes
摘要
Abstract
Objective:To study the protective effect and mechanism of action of Qishen Yiqi Dropping Pills on myocardial ischaemia-reperfusion injury.Methods:Cardiomyocytes were randomly divided into 5 groups,in which group A was normal H9c2 cells+blank serum group,group B was OGD/R+blank serum group,group C was OGD/R+low-dose drug-containing serum group,group D was OGD/R+medium-dose drug-containing se-rum group,and group E was OGD/R+high-dose drug-containing serum group.Corresponding drug interventions were given according to the grouping,and the toxicity of the cell culture fluid was detected by LDH;CCK-8 assay was used to detect cell activity;flow cytometry was used to detect apoptosis;ELISA was used to detect the concen-tration of inflammatory factors;WB was used to detect the expression of p-MST,p-YAP,p-TAZ,TEAD1.Results:Compared with cardiomyocytes in group B,cardiomyocytes in groups C,D and E showed increased activi-ty,decreased apoptosis,decreased cytotoxicity of the culture medium,decreased inflammatory factor content,de-creased expression of p-MST,p-YAP,p-TAZ proteins,and elevated expression of TEAD1 proteins,and the differ-ence was statistically significant(P＜0.01).Conclusion:The protective effect of Qishen Yiqi Dropping Pills on gly-oxylate deprivation/reoxygenation H9c2 cardiomyocytes is closely related to Hippo channel.关键词
心肌缺血再灌注损伤/芪参益气滴丸/H9c2心肌细胞/糖氧剥夺/复氧/Hippo信号通路Key words
Myocardial ischaemia-reperfusion injury/Qishen Yiqi Dropping Pills/H9C2 oardiomyocytes/Glyox-ylate deprivation/reoxygenation/Hippo signaling pathway分类
医药卫生引用本文复制引用
宋惠,何贵新,秦伟彬,杨斐斐,唐伟智,张利文,刘凌云,欧阳彬,朱子一,张清伟..芪参益气滴丸对糖氧剥夺/复氧H9c2心肌细胞的保护作用及机制研究[J].中国中医急症,2025,34(4):593-597,5.基金项目
国家中医药传承创新项目 （）
广西自然科学基金会重点项目(2020GXNSFDA297020) （2020GXNSFDA297020）
研究生教育创新计划项目(YCBXJ2023033) （YCBXJ2023033）
