中国中医急症2025，Vol.34Issue(4)：598-601,615,5.DOI:10.3969/j.issn.1004-745X.2025.04.007
芩百清肺浓缩丸通过TNF-α/NLRP3/Caspase-1信号通路对感染后咳嗽大鼠气道高反应性的影响
Qinbai Qingfei Concentrated Pill's Impact on Airway Hyperresponsiveness in Post-infectious Cough Rats via the TNF-α/NLRP3/Caspase-1 Signaling Pathway
摘要
Abstract
Objective:To observe the impact of Qinbai Qingfei Concentrated Pills(Qinbai)on the TNF-α/NL-RP3/Caspase-1 signaling pathway,thereby alleviating airway hyperresponsiveness in post-infectious cough(PIC)rats.Methods:40 SD healthy male rats were divided into four groups:blank control group,model group,Qinbai group,and positive control group.The models were established using a combination of smoking,LPS solution nasal drops,and capsaicin solution nebulization inhalation.Cough frequency was observed;ELISA was used to measure the content of TNF-α in serum and bronchoalveolar lavage fluid(BALF);HE staining was performed to observe pathological changes in lung tissue;RT-qPCR and Western blotting were used to measure the expression of NL-RP3 and Caspase-1 in lung tissue.Results:Compared with the model group,the frequency of cough significantly decreased in the Qinbai group(P＜0.01);the content of TNF-α in serum and BALF also significantly decreased(P＜0.01);bronchial epithelial injury and inflammatory response were improved;the mRNA levels and protein ex-pression of NLRP3 in lung tissue were significantly reduced(P＜0.01),the mRNA level of Caspase-1 was reduced and its protein expression was significantly reduced(P＜0.05 or P＜0.01).Conclusion:Qinbai Qingfei Concentrat-ed Pills can significantly reduce airway hyperresponsiveness in PIC rats,and its mechanism of action may be relat-ed to the TNF-α/NLRP3/Caspase-1 signaling pathway.关键词
感染后咳嗽/气道高反应性/气道损伤/芩百清肺浓缩丸/TNF-α/NLRP3/Caspase-1信号通路/大鼠Key words
Post-infectious cough/Airway hyperresponsiveness/Airway injury/Qinbai Qingfei Concentrated Pills/TNF-α/NLRP3/Caspase-1 signaling pathway/Rats分类
医药卫生引用本文复制引用
王彬羽,贾维刚,徐玉龙,刘婕妤,张弛,宋小晗,周迎春..芩百清肺浓缩丸通过TNF-α/NLRP3/Caspase-1信号通路对感染后咳嗽大鼠气道高反应性的影响[J].中国中医急症,2025,34(4):598-601,615,5.基金项目
黑龙江省自然科学基金项目(LH2022H059) （LH2022H059）
