中国油料作物学报2025，Vol.47Issue(2)：281-288,8.DOI:10.19802/j.issn.1007-9084.2024206
白菜型油菜-黑芥单体附加系的创制及遗传研究
Development and genetic analysis of momosomic alien addition lines between Brassica rapa and B.nigra
摘要
Abstract
To utilize B genome of Brassica nigra,triploid hybrids obtained from hybridization between B.rapa(turnip-type rapeseed)and black mustard were used as female parents,which were constantly backcrossed with B.rapa in this study.Two B.nigra chromosomes monosomic alien addition lines(MAALs)were identified by molecu-lar markers,cytology and morphology.Progenies′ phenotypes and chromosome behaviors were observed in MAALs.Results showed that B.rapa-nigra B7 and B8 chromosome MAALs exhibited special phenotypes.B7 MAAL exhib-ited severe female infertility,with a light yellow seed coat color and leaves displaying B.nigra trichome phenotype.B8 MAAL exhibited tall plant and multiple first branching from B.nigra.Cytological analysis showed that both B7 and B8 chromosomes of B.nigra existed as univalent during meiosis,and a 45S rDNA locus was found on B7 chro-mosome.Male and female gamete transmission rates revealed that the transmission frequencies of B7 and B8 chro-mosomes were low.B7 chromosome female gamete transmission rate was 9.14%,male gamete transmission rate was 6.58%and B8 chromosome female gamete transmission rate was 20.17%,male gamete transmission rate was 9.05%.B7 and B8 MAALs created in this study were valuable for deeply analysis.关键词
白菜型油菜/黑芥/单体附加系/细胞学/表型Key words
Brassica rapa/Brassica nigra/monosomic alien addition lines/cytology/phenotype分类
农业科技引用本文复制引用
王顺林,李再云,李鹏飞,武海燕,程靖雯,彭梓然,杨芩芩,张密密,谭晨,吴增祥,葛贤宏..白菜型油菜-黑芥单体附加系的创制及遗传研究[J].中国油料作物学报,2025,47(2):281-288,8.基金项目
国家自然科学基金(32101692) （32101692）
贵州省基础研究计划(自然科学)(黔科合基础-ZK[2024]一般381) （自然科学）
贵州中医药大学研究生教育创新计划(YCXKY2023013) （YCXKY2023013）
