中国油料作物学报2025，Vol.47Issue(2)：311-318,8.DOI:10.19802/j.issn.1007-9084.2024016
强抗寒甘蓝型冬油菜愈伤组织体系构建及ROS信号传导规律探究
Callus system construction and ROS signal transduction mechanism of cold resistant winter rape
摘要
Abstract
To understand the mechanism of reactive oxygen species(ROS)in mediating callus development of winter rapeseed(Brassica napus L.),hypocotyl and young leaf of Brassica napus var.16VHNTS309 was used as ex-plants to construct a regeneration system of B.napus.Through observation of reactive oxygen species of O2-signal lo-calization in callus,results showed an optimized system of young leaves and hypocotyls pre culture on MS+1 mg/L or 1.5 mg/L 2,4-D medium for 7 days,respectively.It was followed by MS+3.0 mg/L 6-BA+0.2 mg/L 2,4-D+3 mg/L AgNO3 used as the optimal medium for bud induction.Finally,MS+0.2 mg/L NAA could be used as the rooting medium to obtain good regenerated seedlings of B.napus.Results of ROS(O2-)signal localization showed that a large amount of ROS(O2-)signal was detected in callus cells,meristem cells and cells at the edge of leaves or stems,while ROS(O2-)signal was not detected around cells that had completed bud differentiation or did not form calli,which further indicated that ROS(O2-)signal played an important role in cell division.关键词
甘蓝型油菜/幼叶/下胚轴/愈伤组织/活性氧/信号传导Key words
Brassica napus/young leaf/hypocotyl/callus/reactive oxygen species/signal transmission分类
农业科学引用本文复制引用
祁伟亮,孙万仓,邓素敏,高雪梅,马骊,李妮,张雅,杨财容..强抗寒甘蓝型冬油菜愈伤组织体系构建及ROS信号传导规律探究[J].中国油料作物学报,2025,47(2):311-318,8.基金项目
国家自然科学基金(32360455) （32360455）
陇东学院博士基金项目(XYBYZK2107) （XYBYZK2107）
甘肃省高等学校创新基金项目(2022B-209) （2022B-209）
