中国油料作物学报2025，Vol.47Issue(2)：344-355,12.DOI:10.19802/j.issn.1007-9084.2023321
花生CC-NBS-LRR家族基因鉴定及在青枯病和网斑病胁迫下的表达分析
Genome-wide identification and analysis of CC-NBS-LRR family in response to bacterial wilt and web blotch in peanut
摘要
Abstract
In order to identify the CC-NBS-LRR(CNL)family members and their functions in peanut genome,tbioinformatics analysis and expression pattern of the gene family members were carried out to lay a foundation for peanut responds to disease stress.Using peanut genome sequences as reference,85 AhCNL family members were identified using Pfam,HMMER,NCBI and Coiled Coil software and online sites.AhCNL genes are distributed on 15 out of 20 chromosomes,mainly on A02 and A12.Most of them exist in clusters at chromosomes end.Phylogenetic tree analysis showed that AhCNL genes can be divided into 4 subfamilies,and the gene members in each subfamily are highly conserved.Collinearity analysis showed that there were 16 pairs of genes in the species,and inter-spe-cies analysis showed that CNL of peanut and Glycine max had higher homology than those of Arabidopsis thaliana and Medicago sativa.The promoters of AhCNL genes contain a large number of cis-acting elements related to hor-mone and disease resistance.Most CNL family genes were not expressed before and after being invaded by patho-gens,with a few genes changed after being induced.关键词
花生/CC-NBS-LRR/青枯病/网斑病/表达分析Key words
peanut/CC-NBS-LRR/bacterial wilt/web blotch/gene expression分类
农业科技引用本文复制引用
汪晓,张新友,齐飞艳,孙子淇,郑峥,徐静,王娟,韩锁义,黄冰艳,董文召..花生CC-NBS-LRR家族基因鉴定及在青枯病和网斑病胁迫下的表达分析[J].中国油料作物学报,2025,47(2):344-355,12.基金项目
神农种业实验室一流课题(SN01-2022-03) （SN01-2022-03）
河南省农业科学院杰出青年科技基金(2024JQ06) （2024JQ06）
河南省农科院自主创新项目(2023ZC092) （2023ZC092）
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-13) （CARS-13）
河南省花生产业技术体系(S2012-5) （S2012-5）
优质抗病花生新品种选育(2024TD01) （2024TD01）
