中国油料作物学报2025，Vol.47Issue(2)：356-362,7.DOI:10.19802/j.issn.1007-9084.2023307
花生疮痂病菌次生代谢基因簇特异性转录因子挖掘及EaPSTF1生物信息学分析
Mining of secondary metabolite gene cluster-specific transcription factors and EaPSTF1 bioinformatics of Elsinoë arachidis
摘要
Abstract
Elsinchrome(ESC)is a photosensitive perylene quinone mycotoxin produced by Elsinoë arachidis,which acts as the virulence factor of the pathogen.For deep understanding of the regulatory network governing ESC biosynthesis,we conducted a comprehensive analysis including identification of transcription factors specific to the secondary metabolism gene cluster,EaPSTF1 gene clone,bioinformatics and expression analysis based on whole-genome sequencing.The findings revealed the presence of 3 distinct transcription factors within the pathogen′s sec-ondary metabolism gene cluster.Among them,EaPSTF1 had a length of 1305 bp comprising a complete open read-ing frame,encoding a 110.58 kD-protein with 434 amino acids.And its theoretical isoelectric point was 4.94.Pri-marily located in cell nucleus,EaPSTF1 is a hydrophilic protein dominated by α-helices.It contains 2 Zn(Ⅱ)2Cys6-type domains,GAL4 and AFLR.RT-qPCR analysis demonstrated a consistent expression pattern of EaP-STF1 with toxin accumulation trend,indicating a potential involvement in regulation of ESC toxin biosynthesis.关键词
花生疮痂病菌/特异性转录因子/基因克隆/生物信息学分析/次生代谢基因簇Key words
Elsinoё arachidis/specific transcription factors/gene clone/bioinformatics analysis/secondary metabolite gene cluster分类
农业科学引用本文复制引用
张凌萱,朴静子,刘丹,郝婧文,李自博,周如军..花生疮痂病菌次生代谢基因簇特异性转录因子挖掘及EaPSTF1生物信息学分析[J].中国油料作物学报,2025,47(2):356-362,7.基金项目
辽宁省自然科学基金(2022-MS-264) （2022-MS-264）
辽宁省教育厅面上项目(JYTMS20231291) （JYTMS20231291）
