中国油料作物学报2025，Vol.47Issue(2)：363-369,7.DOI:10.19802/j.issn.1007-9084.2024002
花生籽仁中10种氨基酸含量遗传与QTL定位分析
Genetic analysis and QTL mapping for the contents of 10 amino acids in peanut seed
摘要
Abstract
Amino acid composition is the key of protein quality.To better understand the genetics of peanut amino acid composition,QTL mapping of 10 amino acid contents were carried out by using a RIL population from Jihua 6 and Kaixuan 01-6.Results showed that mean values of the 10 amino acids ranged from 0.24 to 2.42 mg/g.Their phenotypic variation ranged from 4.35%to 11.49%,and their heritability ranged from 0.851 to 0.929,with absolute values of skewness and kurtosis from 0.06 to 1.97.It suggested that the above might be quantitative traits controlled by multiple genes.Most traits of the amino acid contents were significantly correlated,and 4 ami-no acid traits(i.e.valine,isoleucine,leucine and phenylalanine)were closely correlated with the correlation coef-ficient more than 0.91.In addition,23 QTLs associated to 8 amino acids were detected with LOD values ranging from 2.58 to 15.48.One single QTL could explain 2.95%-26.09%phenotype variation,and QTLs for the same amino acid trait could cumulatively explain 8.13%-43.48%phenotype variation.Totally 4 major QTLs contribut-ed more than 10%,namely qMet_6,qLeu_1,qPhe_1 and qHis_1.Near-isogenic line results showed that qMet_6 could significantly increase Met(methionine)content by 32.61%and His(histidine)by 12.70%,indicating that qMet_6 was a major QTL.These were expected to be used on molecular marker-assisted breeding,fine mapping and key gene cloning for peanut.关键词
花生/氨基酸/遗传分析/QTL定位/近等基因系Key words
peanut/amino acid/genetic analysis/QTL mapping/near-isogenic line分类
农业科技引用本文复制引用
郭颂,胡朋举,宋亚辉,金欣欣,苏俏,刘立峰,杨永庆,王瑾..花生籽仁中10种氨基酸含量遗传与QTL定位分析[J].中国油料作物学报,2025,47(2):363-369,7.基金项目
国家现代农业产业技术体系(CARS-13) （CARS-13）
河北省现代农业产业技术体系建设专项(HBCT2023030101、HBCT2023030204、HBCT2023030105) （HBCT2023030101、HBCT2023030204、HBCT2023030105）
河北省现代种业科技创新专项(21326316D) （21326316D）
