中国农业科学2025，Vol.58Issue(8)：1653-1665,13.DOI:10.3864/j.issn.0578-1752.2025.08.015
基于红蓝光调控分析VvBES1-1参与'红地球'葡萄花芽的分化
Analysis of VvBES1-1 Involvement in Flower Bud Differentiation of Red Globe Grape Based on Red and Blue Light Regulation
摘要
Abstract
[Objective]BRI1-EMS-Suppressor 1(BES1),a key transcription factor in brassinosteroid(BR)signaling,regulates plant photomorphogenesis and photoperiodic flowering.This study aimed to investigate the role of BES1 in flower bud differentiation of Vitis vinifera Red Globe under red and blue light regulation,thereby elucidating the BR-mediated mechanisms driven by light quality and providing insights into flowering regulation in other woody fruit trees.[Method]Bioinformatics analysis was performed to characterize the protein structure and sequence alignment of VvBES1-1.Flower buds of Red Globe grape were collected under greenhouse natural light(CK,control)and red:blue=4:1(R4B1)light treatment.qPCR was used to analyze the spatiotemporal expression patterns and tissue specificity of VvBES1-1 during flower bud differentiation.Subcellular localization was determined via tobacco(Nicotiana benthamiana)transient transformation.Protein-protein interactions were examined using bimolecular fluorescence complementation(BiFC),and transcriptional activation activity was assessed via yeast autoactivation assays.[Result]VvBES1-1 contains a BES1_N domain and belongs to the BES1-S-type protein family,showing the closest phylogenetic relationship with Populus trichocarpa BES1.It is expressed throughout all developmental stages of Red Globe grapevines.During R4B1 treatment,the expression level of VvBES1-1 was significantly reduced compared to the control.Its expression peaked during the development of secondary inflorescence axes.Additionally,treatment with 10 mmol·L-¹ EBR enhanced VvBES1-1 expression in Red Globe grape flower buds.Yeast autoactivation assays demonstrated that VvBES1-1 possesses self-activation activity.Subcellular localization analysis revealed that VvBES1-1 is localized in the nucleus.Overexpression of VvBES1-1 in tobacco delayed flowering time,promoted stem elongation,and increased meristem number.Downregulation of VvBES1-1 expression integrated brassinosteroid(BR)and photoperiod signaling pathways to promote flower bud differentiation in Red Globe grapes.During flower bud differentiation,VvBES1-1 expression was significantly regulated by red and blue light.Under R4B1 treatment,its expression peaked after 6 hours of 10 mmol·L-¹ EBR exposure.[Conclusion]VvBES1-1 plays a critical role in flower bud differentiation of Red Globe grapes.It integrates BR and photoperiod signals to inhibit grape flower bud differentiation.However,under red and blue light conditions,the expression of VvBES1-1 is effectively downregulated,thereby promoting flower bud differentiation.This study provides insights into the regulatory mechanisms of light and phytohormones in grape reproductive development.关键词
'红地球'葡萄/VvBES1-1/红蓝光/BR/花芽分化Key words
Red Globe grape/VvBES1-1/red and blue light/BR/bud differentiation引用本文复制引用
汤学燊,党仕卓,周娟,李佳豪,李梅花,胡豪,张亚红..基于红蓝光调控分析VvBES1-1参与'红地球'葡萄花芽的分化[J].中国农业科学,2025,58(8):1653-1665,13.基金项目
宁夏回族自治区重点研发计划(2021BEF2016,2023BCF1054) （2021BEF2016,2023BCF1054）
