中国医学工程2025，Vol.33Issue(4)：17-24,8.DOI:10.19338/j.issn.1672-2019.2025.04.005
线粒体损伤在高糖高脂培养的多巴胺能神经元损伤中的作用研究
Role of mitochondrial damage in dopaminergic neuron damage induced by high glucose and high-fat culture
摘要
Abstract
[Objective]To investigate the proliferation and cell microstructure changes of SN4741 dopamine neuron cells cultured in vitro with high glucose and high fat,and to explore the mechanism of diabetic dopaminergic neuron damage.[Methods]The SN4741 cell line cultured in vitro was divided into two groups:control group and diabetes mellitus(DM)group.The cells were cultured in pure fetal bovine serum medium and DEME medium containing 30 mmol/L high glucose,0.3 mmol/L palmitic acid,and 10%fetal bovine serum for 24 hours,respectively.CCK8 was used to detect cell survival rate,flow cytometry was used to detect cell apoptosis,transmission electron microscopy was used to observe cell microstructure changes,and cell mitochondrial membrane potential was detected.[Results]After adding different concentrations of high glucose,CCK8 detection showed that the survival rate of SN4741 cells in the DM group was significantly decreased,and the degree of decrease was proportional to the high glucose concentration and incubation time,with statistically significant differences(P<0.001).Flow cytometry showed that the apoptosis of cells in the DM group was significantly increased compared with the control group,with statistically significant differences(P<0.05).Transmission electron microscopy showed that the volume of mitochondria in the DM group was enlarged,the mitochondrial ridges were blurred and broken,and some mitochondria were vacuolated.Immunofluorescence showed that compared with the control group,the mitochondrial membrane potential of SN4741 cells cultured in high glucose was reduced.[Conclusion]Diabetes leads to the damage of retinal dopaminergic amacrine cells,and abnormal mitochondrial structure and function may be involved in the occurrence of this cell damage.关键词
糖尿病/多巴胺能无长突细胞/凋亡/增殖/线粒体Key words
diabetes/dopaminergic amacrine cells/apoptosis/proliferation/mitochondria分类
特种医学引用本文复制引用
刘明之,张倬清,张雨鑫,苏建云,杨洋,杨晓春..线粒体损伤在高糖高脂培养的多巴胺能神经元损伤中的作用研究[J].中国医学工程,2025,33(4):17-24,8.基金项目
云南省应用基础研究计划项目(202101AY070001-275) （202101AY070001-275）
云南省兴滇英才项目(YNWR-QNBJ-2018-315) （YNWR-QNBJ-2018-315）
云南省科技人才和平台计划项目(2019HB050) （2019HB050）
内分泌国家级临床重点专科培育项目(2024NMKFKT-02) （2024NMKFKT-02）
