中国医学工程2025，Vol.33Issue(4)：25-30,6.DOI:10.19338/j.issn.1672-2019.2025.04.006
基于改进固相多肽合成技术的前列腺靶向膜抗原的合成
Synthesis of prostate targeted membrane antigen based on improved solid phase peptide synthesis technology
于瀚淼 1徐聪靓 1曹嘉懿 1徐文琪 1张宸瑞 1罗东1
作者信息
- 1. 华南理工大学生物医学科学与工程学院 广州国际校区,广东 广州 511442
- 折叠
摘要
Abstract
[Objective]To optimize the solid-phase peptide synthesis(SPPS)technique in order to solve the problems of decreased synthesis efficiency,increased by-products,and purification difficulties caused by the growth of peptide chains,and to enhance the synthetic efficacy of prostate-targeted membrane antigen C(Trt)Y-PSMA-1.[Methods]Using Fmoc chemical protection strategy,combined with microwave-assisted solid-phase synthesizer and manual synthesis techniques,a peptide chain with sequence Cys-Tyr-(Lys)-Glu-Glu-Glu-Ahx-Amc-Glu'-CO-Glu(n=1,2,4)was constructed step by step using 4-methoxybenzylamino(MBHA)resin as a solid-phase carrier;the peptide chain was cut by acidic conditions to cleave the peptide-resin complexes,and purification and freeze-drying were utilized to obtain the final products by high performance liquid chromatography(HPLC).[Results]The improved method increased the coupling efficiency by 60%and solved the terminal cyclization problem common in the synthesis of long-chain peptides by manual complementary synthesis strategy.[Conclusion]The SPPS technology,which combines microwave-assisted and Fmoc protection,significantly improves the efficiency of long-chain peptide synthesis,and provides a reproducible technological solution for the large-scale preparation of biomaterials for prostate cancer targeting,and the related optimization strategy can be extended to the synthesis of other functional peptides.关键词
固相多肽合成/9-芴甲氧羰基保护基/微波固相多肽合成仪/前列腺靶向膜抗原Key words
solid-phase peptide synthesis/Fmoc chemistry/microwave solid-phase peptide synthesizer/prostate targeted membrane antigen分类
药学引用本文复制引用
于瀚淼,徐聪靓,曹嘉懿,徐文琪,张宸瑞,罗东..基于改进固相多肽合成技术的前列腺靶向膜抗原的合成[J].中国医学工程,2025,33(4):25-30,6.
