中国油料作物学报2025，Vol.47Issue(2)：421-434,14.DOI:10.19802/j.issn.1007-9084.2024038
BnaNF-YA2增强甘蓝型油菜渗透胁迫抗性的功能鉴定
Functional characterization of BnaNF-YA2 in enhancing osmotic stress resistance in Brassica napus
摘要
Abstract
Rapeseed(Brassica napus L.)is one of the main sources of edible vegetable oil in China.Nuclear factor Y(NF-Y)proteins comprise a very important transcription factor family in plants,and its key component NF-YA usually functions with NF-YB/NF-YC dimer to form a trimer,which plays an important role in plant develop-ment and stress response.To better understand the function of BnaNF-YA family members in response to abiotic stress,and to provide a theoretical basis for cultivating rapeseed materials with improved stress resistance,we ana-lyzed BnaNF-YA2 in response to abiotic stress by bioinformatics and expression patterns,by overexpressing BnaNF-YA2 in B.rapus and identifying their stress phenotypes.Identification results of cis-elements showed that the BnaNF-YA2 promoter region were rich in stress-related response elements.The expression analysis revealed that PEG,heat and low temperature induced BnaNF-YA2 expression.Results also indicated that BnaNF-YA2 over-expression might enhance the growth of B.napus under PEG and mannitol treatments during the post-germination stage.BnaNF-YA2 positively regulated the tolerance of B.napus to osmotic stress.关键词
甘蓝型油菜/转录因子/NF-YA/渗透胁迫/表型鉴定Key words
Brassica napus/transcription factors/NF-YA/osmotic stress/phenotypic identification分类
农业科学引用本文复制引用
王高升,王佩瑶,孙念礼,王幼平,方玉洁..BnaNF-YA2增强甘蓝型油菜渗透胁迫抗性的功能鉴定[J].中国油料作物学报,2025,47(2):421-434,14.基金项目
国家自然科学基金(32171969) （32171969）
江苏省农业科技自主创新资金(CX(22)3085) （CX(22）
