针灸和草药（英文）2025，Vol.5Issue(1)：76-88,13.DOI:10.1097/HM9.0000000000000149
基于UHPLC-QTOF MS的蓝芩口服液大鼠体内代谢产物表征
Characterization of metabolic profiles of Lanqin Oral Liquid in rats by ultra-high-performance liquid chromatography tandem time-of-flight mass spectrometry
谭粤玥 1王梦圆 1范语诺 1范雅柳 1张晔 1李彬 1王永香 2杨华 1李萍1
作者信息
- 1. 中国药科大学,中药学院,多靶标天然药物全国重点实验室,南京
- 2. 南京海陵中药制药工艺技术研究有限公司,南京
- 折叠
摘要
Abstract
Objective:Lanqin oral liquid(LOL),as a traditional Chinese medicine prescription,has obvious clinical efficacy in the treatment of pharyngeal inflammation.Exploring the distribution of LOL prototype components and metabolites in plasma is of great significance for understanding potentially effective compounds.The aim of this study is to elucidate the metabolites and main metabolic pathways of LQL in vivo.
Methods:In this study,a reliable approach integrated background subtraction and mass defect filtering(MDF),based on quadrupole time-of-flight mass spectrometry(QTOF-MS)technology,was performed to systematically scan the metabolites of LOL in rat plasma.In addition,according to the prototype mass spectrometry fragmentation pattern and combined with metabolic pathway analysis,a biotransformation oriented analysis strategy was established and applied to the identification of metabolites in LOL in vivo.
Results:As a result,159 compounds(58 prototypes and 101 metabolites)were identified or tentatively characterized in drug-containing plasma,including 74 flavonoids,30 alkaloids,34 terpenoids,five phenylpropanoids,six phenolic acids,five fatty acids,and five other type components.The main metabolic pathways include methylation,demethylation,hydroxylation,hydrogenation,glucuronidation,and sulfation.
Conclusions:This study provides an overall characterization of the metabolites of LOL in vivo for the first time,providing a solid material basis for exploring the therapeutic effects and pharmacological mechanisms of LOL.关键词
蓝芩口服液/代谢物表征/质量亏损过滤/UHPLC-QTOF MS/背景扣除Key words
Background subtracts/Lanqin Oral Liquid/Mass defect filtering/Metabolic profiles/UHPLC-QTOF-MS引用本文复制引用
谭粤玥,王梦圆,范语诺,范雅柳,张晔,李彬,王永香,杨华,李萍..基于UHPLC-QTOF MS的蓝芩口服液大鼠体内代谢产物表征[J].针灸和草药（英文）,2025,5(1):76-88,13.
