针灸和草药（英文）2025，Vol.5Issue(1)：103-114,12.DOI:10.1097/HM9.0000000000000145
电针对荨麻疹大鼠模型肠道微生物群的影响
Effects of electroacupuncture on gut microbiota in a rat model of urticaria
肖显俊 1薛佩雯 1秦海燕 2秦迪 3王路 4金荣疆 1李瑛 3石云舟 3李涓5
作者信息
- 1. 成都中医药大学养生康复学院,成都
- 2. 深圳大学第一附属医院针灸科,成都
- 3. 成都中医药大学针灸推拿学院,成都
- 4. 成都中医药大学附属医院针灸科,成都
- 5. 成都中医药大学养生康复学院,成都||成都中医药大学附属康复医院,成都
- 折叠
摘要
Abstract
Objective:Growing evidence suggests a strong link between gut microbiota and the pathogenesis of urticaria,positioning the gut-skin axis as a novel therapeutic target.Despite the efficacy of acupuncture in alleviating urticaria symptoms,the underlying mechanisms remain poorly understood.We investigated the effects of electroacupuncture on gut microbiota in a rat model of urticaria.
Methods:Sprague-Dawley rats were randomly divided into three groups:control,model,and electroacupuncture.An urticaria model was established by combining active systemic and passive cutaneous allergic reactions.Electroacupuncture treatment was administered for 7 days,during which the number of scratches,the diameter of skin sensitization blue spots,and degranulation rate of mast cells were recorded.Inflammation in the sensitized skin was assessed using hematoxylin and eosin staining,and the composition of the gut microbiota was analyzed using 16S rRNA gene sequencing.
Results:Electroacupuncture significantly reduced the frequency of scratching,the diameter of sensitized blue spots,and the degranulation rate of mast cells in rats.The staining results revealed that electroacupuncture decreased inflammatory cell infiltration and fibrous tissue proliferation in the sensitized skin.The 16S rRNA gene sequencing demonstrated that electroacupuncture adjusted the ratio of Firmicutes to Bacteroidetes.Receiver operating characteristic(ROC)curve analysis identified Parabacteroides johnsonii as a potential biomarker for diagnosing urticaria(AUC=0.8516,where AUC stands for the area under the curve)and Lactobacillus reuteri and Limosilactobacillus as potential biomarkers for predicting the effectiveness of acupuncture treatment(AUC=0.8281).
Conclusion:Electroacupuncture may alleviate urticaria symptoms by modulating the gut microbiota,providing new theoretical and practical directions for acupuncture treatment of urticaria.关键词
电针/厚壁菌门/拟杆菌门比例/肠道菌群/肠道-皮肤轴/荨麻疹Key words
Electroacupuncture/Firmicutes/bacteroidetes ratio/Gut microbiota/Gut-skin axis/Urticaria引用本文复制引用
肖显俊,薛佩雯,秦海燕,秦迪,王路,金荣疆,李瑛,石云舟,李涓..电针对荨麻疹大鼠模型肠道微生物群的影响[J].针灸和草药（英文）,2025,5(1):103-114,12.
