中国医科大学学报2025，Vol.54Issue(4)：369-374,6.DOI:10.12007/j.issn.0258-4646.2025.04.015
芦丁促进人乳牙牙髓干细胞的增殖及成骨分化
Rutin promoting the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth
摘要
Abstract
Objective To investigate the effects of rutin on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHEDs)and its regulatory relationship with the Janus protein tyrosine kinase 2(JAK2)/signal transducer and activator transcription 3(STAT3)pathway.Methods SHEDs were isolated and cultured in vitro.After identification,SHEDs were randomly grouped into the control group(normal culture),low,medium,and high dose rutin groups(0.1,0.5,1 µmol/L),and the inhibi-tor group(1 μmol/L of rutin+10 μmol/L of the JAK2/STAT3 pathway inhibitor AG490).Alizarin red staining was applied to detect the number of mineralized nodules,and the MTT method was utilized to detect cell proliferation.A commercial kit was used to detect cell alkaline phosphatase(ALP)activity,and quantitative real-time PCR was applied to detect the mRNA expression of Runt-related transcrip-tion factor 2(Runx2),osteocalcin(OCN),and alkaline phosphatase(ALP).Western blotting was used to detect the expression of Runx2,OCN,ALP,and JAK2/STAT3 signaling pathway proteins.Results Compared to that of the control group,the optical density value of SHEDs cells,the number of mineralized nodules,ALP activity,Runx2,OCN,ALP mRNA and protein levels,and p-JAK2/JAK2 p-STAT3/STAT3 were all markedly increased in the low,medium,and high dose rutin groups(P＜0.05).Treatment with the JAK2/STAT3 pathway inhibitor AG490 attenuated the enhancement of rutin on the proliferation and osteogenic differentiation of SHEDs(P＜0.05).Conclu-sion Rutin promotes the proliferation and osteogenic differentiation of SHEDs,and its mechanism is related to the activation of the JAK2/STAT3 signaling pathway.关键词
芦丁/Janus蛋白酪氨酸激酶2/信号转导及转录激活蛋白3/人乳牙牙髓干细胞/增殖/成骨分化Key words
rutin/Janus protein tyrosine kinase 2/signal transducer and activator of transcription 3/stem cells from human exfoliated deciduous teeth/proliferation/osteogenic differentiation分类
医药卫生引用本文复制引用
冯妮,李月红..芦丁促进人乳牙牙髓干细胞的增殖及成骨分化[J].中国医科大学学报,2025,54(4):369-374,6.基金项目
湖南创新型省份建设专项(2020ZK4073) （2020ZK4073）
