针灸临床杂志2025，Vol.41Issue(3)：74-79,6.DOI:10.19917/j.cnki.1005-0779.025055
穴位电刺激对大鼠肝纤维化损伤的保护作用
Protective Effect of Acupoints Electrical Stimulation on Hepatic Fibrosis Injury in Rats
摘要
Abstract
Objective:To observe the protective effects of electro-needling Zusanli(ST36),Ganshu(BL18),Sanyinjiao(SP6),Guanyuan(RN4)and Taichong(LR3)on hepatic fibrosis injury in rats,and to explore its mechanisms.Methods:36 SPF grade male SD rats were randomly divided into the blank group,the model group,and the electro-acupuncutre(EA)group,with 12 rats in each group.The hepatic fibrosis model was established by CCL4 olive oil solution.The treatment group received electrical stimulation treatment on ST36,BL18,SP6,RN4 and LR3 one day after modeling,with each intervention lasting 15 minutes,once a day for 21 consecutive days.After the completion of modeling and 21 days of intervention,samples were taken from the liver tissues of rats in each group.The pathological changes and degree of liver fibrosis were observed using HE staining and Masson staining.ELISA method was used to detect the serum levels of hyaluronic acid(HA),laminin(LN),and type Ⅲ procollagen(PC Ⅲ).Real time fluorescence quantitative PCR was used to detect the relative expressions of α-SMA and Collagen1 gene mRNA in liver tissues.Results:HE staining and Masson staining showed that a large number of liver cells occurred in the model group,and there were accumulations of necrosis and collagen fiber accumulation.In the treatment group,collagen fiber accumulation decreased and the liver cell structure tended to be normal.Compared with those in the blank group,the ELISA method detected that the serum levels of HA,LN and PC Ⅲ significantly increased in the model group;compared with those in the model group,the serum levels of HA,LN and PC Ⅲ decreased in the EA group.Real time fluorescence quantitative PCR experiment validated the contents of α-SMA and Collagen1 mRNA increased(P＜0.05),which proved that the model was successfully established,and the contents of α-SMA and Collagen1 mRNA significantly decreased in the treatment group(P＜0.05).Conclusion:Electro-needling ST36,GL18,SP6,RN4,and LR3 has a certain protective effect on hepatic fibrosis damage.关键词
肝纤维化/电针/α-SMA/Collagen1Key words
Hepatic fibrosis/Electro-acupuncture/α-SMA/Collagen1分类
医药卫生引用本文复制引用
秦霈瑶,胡晓阳..穴位电刺激对大鼠肝纤维化损伤的保护作用[J].针灸临床杂志,2025,41(3):74-79,6.基金项目
国家自然科学基金,编号:81202638 （）
全国名中医专家传承工作室项目,编号:14061220023. （）
