中国输血杂志2025，Vol.38Issue(4)：514-521,8.DOI:10.13303/j.cjbt.issn.1004-549x.2025.04.009
基于血小板代谢组学探究4℃冷藏对单采血小板保存的影响
Research the effect of 4℃refrigerated stored apheresis platelets based on platelet metabolomics
摘要
Abstract
Objective To investigate the differences in metabolomics between apheresis platelets stored at 4℃and at 22℃with agitation,aiming to provide a theoretical basis for the cold storage of apheresis platelets.Methods Samples were collected at four time points(d1,d5,d10,d15)for platelets stored at 4℃(experimental group)and two time points(d1,d5)for platelets stored at 22℃with agitation(control group).Liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology was used to detect changes in platelet metabolome levels under different storage conditions.Plate-let functional activity was assessed by thromboelastography(TEG)for maximum amplitude(MA)values and flow cytometry for CD62P activation rates.Results Metabolites in the glycolytic pathway,key metabolites in the tricarboxylic acid cycle(citrate,α-ketoglutarate),metabolites in the purine metabolism pathway(adenine,inosine monophosphate,guanine,etc.)and amino acid metabolites significantly decreased by d5 in the control group,whereas they remained stable in the ex-perimental group.The content of fatty acid metabolites,such as prostaglandin G2,13(S)-HOTrE,and linoleic acid,sig-nificantly increased in the control group.Statistically significant differences in MA values were observed between the two groups at d1 and d5(P<0.05).However,in the experimental group,as the storage time extended,the MA values at d10 and d15 showed no significant difference compared to the control group at d5(P>0.05).The CD62P activation rate be-tween the two groups was statistically significant(P<0.05).Additionally,the CD62P activation rate of platelets in the 22℃group increased rapidly from d1,while it rose gradually in the 4℃group.Conclusion Platelets stored at 4℃exhibit more stable metabolic activity and slower functional deterioration,which is beneficial for extending the effective storage peri-od of platelets.关键词
冷藏保存/单采血小板/代谢组学Key words
refrigerated storage/apheresis platelets/metabolomics分类
临床医学引用本文复制引用
夏小叶,李雪晶,苏爱华,郝萧,叶红艳..基于血小板代谢组学探究4℃冷藏对单采血小板保存的影响[J].中国输血杂志,2025,38(4):514-521,8.基金项目
济南市卫生健康委员会科技计划项目(2023-1-64) （2023-1-64）
