照明工程学报2025，Vol.36Issue(2)：39-48,10.DOI:10.3969/j.issn.1004-440X.2025.02.007
630 nm发光二极管照射脂多糖刺激的THP-1来源巨噬细胞的多组学分析
The Mult-Omics Analysis of THP-1-Derived Macrophages Stimulated by 630 nm Light Emitting Diodes and Lipopolysaccharide
摘要
Abstract
LED,as a form of Low-Level Light Therapy(LLLT),have been proven effective in treating diseases such as lung injury,tendinopathy,nerve damage,and rheumatoid arthritis.This study uses 630 nm LED red light as the light source to analyze the mechanism of action of 630 nm LED red light on macrophages derived from human monocytic leukemia cells(THP-1)stimulated with lipopolysaccharides(LPS).THP-1 cells were treated with LPS and irradiated with 630 nm LED red light at an intensity of 28.8 J/cm2.The mRNA expression levels of pro-inflammatory factors CXCL9,CXCL10 and CXCL11 were then measured.Differentially expressed proteins were screened using non-quantitative proteomics and phosphoproteomics analysis methods.Gene Ontology(GO)and KEGG enrichment analyses were performed.Through STRING online database(https://cn.string-db.org/)and Cytoscape software is used to analyze protein interactions.The results showed that the mRNA expression levels of CXCL9,CXCL10 and CXCL11 in the LED-irridated THP-1 cells were significantly reduced compared to the control group.Proteomic analysis revealed that after 630 nm LED red light irradiation of macrophages,23 differentially expressed proteins were significantly enriched in pathways such as transcriptional regulation complexes and viral defense response regulation.Phosphoproteomics results showed that the modification levels of 178 sites were significantly enriched in pathways such as nuclear spots,cadherin binding,protein signal transduction,positive regulation of protein localization,and actin cytoskeleton.Combined with multi-omics analysis,we speculate that the 630 nm LED red light may exert anti-inflammatory effects through multiple pathways.关键词
发光二极管/THP-1/630 nm红光/蛋白质组学/磷酸化蛋白质组学/光生物调节Key words
light-emitting diode/THP-1/630 nm red light/proteomics/phosphorylated proteomics/photobiological regulation分类
信息技术与安全科学引用本文复制引用
任丹丹,毕婉莹,谢文婷,吴佳欣,宋武琦,张凤民,刘海亮..630 nm发光二极管照射脂多糖刺激的THP-1来源巨噬细胞的多组学分析[J].照明工程学报,2025,36(2):39-48,10.基金项目
国家重点研发计划资助项目(2022YFB3604705,2017YFB0403805) （2022YFB3604705,2017YFB0403805）
国家自然科学基金项目(81701573) （81701573）
黑龙江省自然科学基金优秀青年项目(YQ2020H004) （YQ2020H004）
