作物学报2025，Vol.51Issue(5)：1400-1408,9.DOI:10.3724/SP.J.1006.2025.44145
花生开花时间的全基因组关联分析及候选基因筛选
Genome-wide associate analysis of flowering traits and identification of candi-date genes in peanut
摘要
Abstract
The flowering time,one of the important indexes to evaluate the early maturing varieties of peanut,has an important impact on the yield.It is very important to mine the genetic loci related to peanut flowering time and screen candidate genes for early maturation breeding of peanut.In this paper,the flowering time of 390 peanut germplasms in five environments was evalu-ated.Phenotypic statistics showed that the variation coefficient of peanut flowering time ranged from 3.21%to 8.54%in five en-vironments,and there were abundant phenotypic variations.The flowering time of peanut basically showed normal distribution and was greatly affected by the environment.A total of 259 SNPs significantly associated with flowering time were identified by genome-wide association analysis.Of them,29 were identified in more than two environments,and located on chromosomes A01,A02,A03,A04,A05,A07,A10,B01,B02,B03,B04,B06,B07,and B09,it can explain 3.47%to 8.58%of the phenotypic varia-tion.After searching for candidate genes in the confidence intervals of 29 recurrent loci upstream and downstream 100 kb,159 genes with functional annotations were found.Seven candidate genes related to peanut flowering time were predicted in the candidate interval,encoding R2R3-MYB transcription factor,chlorophyll a-b binding protein,bHLH transcription factor,WRKY transcription factor and FAR1 transcription factor,respectively.The results of this study can lay a foundation for understanding the genetic mechanism of peanut flowering regulation and peanut early maturation breeding.关键词
花生/开花时间/全基因组关联分析/候选基因/早熟育种Key words
peanut/flowering time/genome-wide association analysis/candidate gene/early maturation breeding引用本文复制引用
李文佳,廖泳俊,黄璐,鲁清,李少雄,陈小平,金晶炜,王润风..花生开花时间的全基因组关联分析及候选基因筛选[J].作物学报,2025,51(5):1400-1408,9.基金项目
本研究由广州市基础与应用基础研究项目(2023A04J0776),广东省农业科学院农业优势产业学科团队项目(202104TD)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13)资助. This study was supported by the Guangzhou Basic and Applied Basic Research Foundation(2023A04J0776),the Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences(202104TD),and the China Agriculture Research System of MOF and MARA(CARS-13). （2023A04J0776）
