摘要
Abstract
Objective To explore the effect of miR-338-5p on proliferation,apoptosis and osteogenic differentiation of alveolar bone osteoblasts by targeting Runt-related transcription factor 2(Runx2).Methods Human alveolar bone osteoblasts were isolated and transfected,Osteoblasts divided into Overexpression of miR-338-5p group(miR-338-5p group)and its control group(miR-NC group),overexpression of Runx2 group(Runx2 group)and its control group(NC group),overexpression of miR-338-5p+Runx2 group(miR-338-5p+Runx2 group)and its control group(miR-338-5p+NC group).The dual luciferase report experiment was used to verify the regulatory effect of miR-338-5p on Runx2;cell counting kit-8(CCK-8)and 5-Ethynyl-2'-deoxyuridine(EdU)staining were used to detect cell proliferation ability;AnexinV FITC/PI method was used to detect cell apoptosis ability;Alkaline phosphatase(ALP)staining was used to detect cell osteogenic differentiation ability;RT-qPCR was used to detect miR-338-5p,Runx2 mRNA levels;Western blot was used to detect Runx2,proliferating cell nuclear antigen(PCNA),Ki67,B-cell lymphoma/leukemia-2(Bcl-2),Bcl-2 related X protein(Bax),ALP,osteocalcin(OCN),osteopontin(OPN)levels.Results miR-338-5p targeted Runx2.After overexpression of miR-338-5p,cell viability,EdU positive cell rate and osteogenic differentiation ability were decreased,Apoptosis rate was increased.miR-338-5p,Bax protein levels were increased,Runx2 mRNA and protein levels,PCNA,Ki67,Bcl-2,ALP,OCN,OPN protein levels were decreased(P＜0.05).After overexpression of Runx2,cell viability,EdU positive cell rate and osteogenic differentiation ability were increased,Apoptosis rate was decreased.miR-338-5p,Bax protein levels were decreased,Runx2 mRNA and protein levels,PCNA,Ki67,Bcl-2,ALP,OCN,OPN protein levels were increased(P＜0.05);Overexpression of Runx2 could weaken the effects of overexpression of miR-338-5p on cell proliferation,apoptosis and osteogenic differentiation(P＜0.05).Conclusion miR-338-5p inhibits alveolar bone osteoblast proliferation and osteogenic differentiation,and promots alveolar bone osteoblast apoptosis by targeting Runx2.关键词
miR-338-5p/Runt-相关转录因子2(Runx2)/牙槽骨成骨细胞/增殖/成骨分化Key words
miR-338-5p/Runt-related transcription factor 2(Runx2)/alveolar bone osteoblast/proliferation/osteogenic differentiation分类
医药卫生
