中药材2024，Vol.47Issue(10)：2428-2433,6.DOI:10.13863/j.issn1001-4454.2024.10.004
芍药黑斑病病原菌的分离鉴定、生物学特性及植物源药剂筛选
Identification,Biological Characteristics and Botanical Pesticide Screening of the Pathogen of Black Spot on Paeonia lactiflora
摘要
Abstract
Objective:To clarify the pathogen species and biological characteristics of black spot disease of Paeonia lactiflora in Xinyang area,and screen the suitable botanical pesticide.Methods:The Paeonia lactiflora diseased leaves were collected from the plant-ing area,the pathogenic bacteria were isolated and purified in the laboratory,and the pathogenic back inoculation test was carried out.The pathogenic strains were identified by morphology and molecular biology.By studying the biological characteristics of the pathogen,the optimal culture conditions were determined.The toxicity of three botanical pesticides to black spot of Paeonia lactiflora was deter-mined by mycelial growth rate method.Results:Combined with the analysis of morphology and molecular biology identification based on rDNA-ITS sequence and tef1 genes,the pathogen causing black spot disease of Paeonia lactiflora in Xinyang area was Alternaria alter-nata.The optimum culture conditions for the pathogen were 28℃,pH 9,and the optimum carbon and nitrogen sources for growth were starch and potassium nitrate,respectively.The EC50 of 1%osthole AS,0.5%physcion AS and 5%allicin ME were 19.772,243.245 and 1 810.685 mg/L,respectively.Conclusion:The pathogen of black spot disease of Paeonia lactiflora in Xinyang area is Alternaria al-ternata.The optimum culture condition of the pathogen is temperature 28℃,pH 9,and the optimum carbon and nitrogen sources are starch and potassium nitrate,respectively,1%osthole AS has a strong inhibitory effect on the pathogen.关键词
芍药/交链格孢菌/生物学特性/植物源农药Key words
Paeonia lactiflora Pall./Alternaria alternata/Biological characteristics/Botanical pesticide分类
植物保护学引用本文复制引用
谷清义,黄晨晨,武志鹏,张耀洲,黄雅琴,申君..芍药黑斑病病原菌的分离鉴定、生物学特性及植物源药剂筛选[J].中药材,2024,47(10):2428-2433,6.基金项目
2021年度河南省高等学校重点科研项目(21B210009) （21B210009）
河南省科技攻关项目(222102110247) （222102110247）
信阳农林学院青年教师科研基金项目(QN2021060) （QN2021060）
