中药材2024，Vol.47Issue(10)：2466-2470,5.DOI:10.13863/j.issn1001-4454.2024.10.010
基于简化基因组的金刚藤群体结构和遗传多样性研究
Study on Population Structure and Genetic Diversity of Smilax china Based on Simplified Genome
摘要
Abstract
Objective:To understand the population structure and genetic diversity of Smilax china from different places,so as to provide scientific basis for better exploitation and utilization of Smilax china,a genuine medicinal resource in Hubei province.Methods:The population structure,principal component analysis,phylogenetic relationship and genetic diversity index of 36 samples from five provinces were analyzed one by one based on the sequencing of SNP sites in simplified genomes.Results:The samples were divided into G1 and G2 groups.The average expected heterozygosity(He)and observed heterozygosity(Ho)of the two groups were 0.0608 and 0.0611,respectively.The inbreeding coefficients(Fis)were 0.4434 and 0.4344,respectively.The average polymorphism information content(PIC)was 0.0497 and 0.0477,respectively.Nucleic acid diversity(π)was 0.0622 and 0.0705,respectively.The population differentiation index(Fst)was 0.8191.Conclusion:The 36 samples collected can be divided into two groups,the degree of genetic dif-ferentiation between the two groups is large,but the genetic distance within each group is small.This study lays a solid foundation for further germplasm evaluation,breeding population construction and new variety breeding.关键词
金刚藤/简化基因组测序/遗传多样性/群体结构Key words
Smilax china L./Simplified genome sequencing/Genetic diversity/Population structure分类
中医学引用本文复制引用
吴高岭,吴和鸣,李名钢,王潜,左岚,宋晓芳,王志勇..基于简化基因组的金刚藤群体结构和遗传多样性研究[J].中药材,2024,47(10):2466-2470,5.基金项目
中央引导地方科技发展基金项目(Z135050009017-429) （Z135050009017-429）
