中药材2024，Vol.47Issue(10)：2494-2498,5.DOI:10.13863/j.issn1001-4454.2024.10.015
无蹼壁虎内生菌Graphium sp.GD-11次级代谢产物研究
Study on the Secondary Metabolites from Intestinal Endophytes Graphium sp.GD-11 of Gekko swinhonis
摘要
Abstract
Objective:To study the secondary metabolites of Graphium sp.GD-11,a stickleback strain isolated from the intestinal endophytes of Gekko swinhonis.Methods:The Graphium sp.strain was fermented with rice medium and the fermentation products were extracted by ethyl acetate and isolated and purified by silica gel column,ODS column,Sephadex LH-20 and semi-preparative liquid chromatography,the compounds were identified by spectral data.Results:Ten compounds were isolated from intestinal endophytes Graphium sp.GD-11 of Gekko swinhonis and identified as 6-(α-L-rhamnopyranosyl)-p-cymene-2,3-diol(1),epi-guaidiol A(2),malas-sezione(3),N-(2'-hydroxytetracosyl)-1,3,4-trihydroxy-2-amino-octadecane(4),p-hydroxybenzaldehyde(5),p-hydroxyphenylacetic acid methyl ester(6),dibutyl phthalate(7),4-hydroxyphenethyl-2-(4-hydroxyphenyl)acetate(8),(2S)-1-O-heptatriacontanoyl glycer-ol(9),(+)-ethyl-4-methylheptanoate(10),respectively.Conclusion:Among them,compound 1 is a new compound,and compounds 2 to 10 are isolated from secondary metabolites of Graphium sp.GD-11 for the first time.Compound 1 can inhibit the release of NO from RAW 264.7 cells induced by LPS.关键词
无蹼壁虎/粘束孢属/次级代谢产物/分离鉴定Key words
Gekko swinhonis Günther/Graphium sp./Secondary metabolites/Isolation and identification分类
医药卫生引用本文复制引用
吴易勋,张明月,张丽萍,任建南,刘水新,郭涛..无蹼壁虎内生菌Graphium sp.GD-11次级代谢产物研究[J].中药材,2024,47(10):2494-2498,5.基金项目
河南省高层次人才国际化培养资助项目(豫科[2021]72号) （豫科[2021]72号）
