中药材2024，Vol.47Issue(10)：2505-2509,5.DOI:10.13863/j.issn1001-4454.2024.10.017
参环毛蚓的化学成分和神经保护活性研究
Chemical Constituents of Pheretima aspergillum and Neuroprotective Activities
摘要
Abstract
Objective:To study the chemical constituents of Pheretima aspergillum and neuroprotective activities.Methods:Silica gel,Sephadex LH-20 dextran gel,ODS column and semi-preparative liquid chromatography were used to isolate and purify the chemical components of Pheretima aspergillum.The structures of the compounds were identified by MS,1 H-NMR,13 C-NMR and other modern spectral techniques.The neuroprotective activities of the compounds were evaluated by 6-hydroxydopamine(6-OHDA)-induced SH-SY5Y cell injury model.Results:Fifteen compounds were isolated from the ethanol extract of Pheretima aspergillum and identified as er-gosta-7,22-diene-3β,5α,6α-triol(1),cholesterol(2),cyclo(L-Pro-L-Val)(3),cyclo(L-Pro-L-Ile)(4),cyclo(L-Pro-L-Leu)(5),pericochlorosin A(6),benzoic acid(7),4-hydroxybenzeneacetamide(8),catechol(9),p-dihydroxybenzene(10),uracil(11),thymine(12),6,9,10-trihydroxy-octadec-7-enoic acid(13),palmitic acid(14),methyl linoleate(15),respectively.Conclusion:Above com-pounds are isolated from Pheretima aspergillum for the first time,compounds 1,3,and 4 can inhibit 6-OHDA-induced SH-SY5Y cell in-jury and show significant neuroprotective activities.关键词
参环毛蚓/化学成分/神经保护活性Key words
Pheretima aspergillum(E.Perrier)/Chemical constituents/Neuroprotective activities分类
医药卫生引用本文复制引用
虞寅杰,国欢,樊青青,任文硕,吴霞,郭强,陈筱清..参环毛蚓的化学成分和神经保护活性研究[J].中药材,2024,47(10):2505-2509,5.基金项目
国家自然科学基金青年科学基金项目(22207078) （22207078）
北京市教育委员会科技计划一般项目(KM202310025017) （KM202310025017）
