浙江大学学报（理学版）2025，Vol.52Issue(3)：312-322,11.DOI:10.3785/j.issn.1008-9497.2025.03.003
基于TMT标记的定量蛋白质组学分析超低温冷冻对小黄鱼肌肉差异蛋白质的影响
TMT labeling based Quantitative proteomics analysis for the effect of ultra-low temperature freezing on the muscle differential proteins of small yellow croaker(Larimichthys polyactis)
摘要
Abstract
In order to investigate the effect of ultra-low temperature(-45℃)freezing time on the differential protein expression of small yellow croaker(Larimichthys polyactis)muscle,quantitative proteomics technology with TMT label was used.The results showed that compared with fresh yellow croaker,there were 71 and 104 different proteins in the muscle after 3 and 6 months of cryopreservation,including 13 and 40 up-regulated proteins and 58 and 64 down-regulated proteins,respectively.More protein changes were found in the comparison between 0 and 6 months compared with the difference between 0 and 3 months.In addition,a total of 15 different proteins were selected,which were significantly correlated with at least one of the quality indexes of pH value,water-holding capacity,TVBN,hardness,springiness,as well as carbonyl content,total sulfhydryl and Ca2+-ATPase activities of small yellow croaker(Larimichthys polyactis),and were expected to be indicator protein markers related to the quality of frozen small yellow croaker.关键词
超低温冷冻/蛋白质组学/差异蛋白/小黄鱼Key words
ultra-low temperature freezing/proteomics analysis/differential proteins/Larimichthys polyactis分类
轻工业引用本文复制引用
许丹,马新悦,郑斌,邓尚贵,张小军..基于TMT标记的定量蛋白质组学分析超低温冷冻对小黄鱼肌肉差异蛋白质的影响[J].浙江大学学报（理学版）,2025,52(3):312-322,11.基金项目
"十三五"国家重点研发计划项目(2020YFD0900900) （2020YFD0900900）
舟山市科技计划项目(2021C31010). （2021C31010）
