中药材2024，Vol.47Issue(10)：2514-2519,6.DOI:10.13863/j.issn1001-4454.2024.10.019
巴西甘菊花化学成分及其体外抗非小细胞肺癌活性研究
Chemical Constituents of Achyrocline satureioides and the Inhibition on Non-Small Cell Lung Cancer In Vitro
摘要
Abstract
Objective:To investigate the chemical constituents of Achyrocline satureioides and their inhibition on non-small cell lung cancer in vitro.Methods:The extract of Achyrocline satureioides was isolated and purified by ODS,Sephadex LH-20 and semi-preparative HPLC,the structures of the isolated compounds were identified by the spectroscopic methods of nuclear magnetic resonance and mass spectrum,etc.The inhibition on non-small cell lung cancer in vitro of the compounds in Achyrocline satureioides was determined by the CCK8.Results:Eighteen compounds were isolated from Achyrocline satureioides and identified as grandifloric acid(1),4,2',4',β-tetra-hydroxy-6'-methoxy-α,β-dihydrochalcone(2),oplopanone(3),broussonin A(4),hinokiresinol(5),benzoic acid(6),2',3-dihydroxy-5-methoxybibenzyl(7),2,4,6-trimethoxyphenol(8),4,2',4'-trihydroxy-6'-methoxydihydrochalcone(9),3-methylkaempferol(10),1-methylbenzimidazole(11),3,5-dimethoxybenzaldehyde(12),isosakuranetin(13),umbelliferone(14),norauricepyrone(15),ent-kaur-16-en-18-ol(16),lepidissipyrone(17),ursolic acid(18),respectively.Compounds 1,2,3,5,7,9,10,17,18 inhibited the proliferation of A549 cells in vitro.Conclusion:Among them,compounds 1 to 16 are isolated from Achyrocline satureioides for the first time.Com-pounds 1,2,3,5,7,9,10,17,18 show inhibition of non-small cell lung cancer at 100 μmol/L in vitro.关键词
巴西甘菊花/化学成分/非小细胞肺癌/CCK8Key words
Achyrocline satureioides(Lam.)DC./Chemical constituents/Non-small cell lung cancer/CCK8分类
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彭佳鹂,刘宏栋,彭富华,皮梦婷,蒋涛,韩晓毓,李斌..巴西甘菊花化学成分及其体外抗非小细胞肺癌活性研究[J].中药材,2024,47(10):2514-2519,6.基金项目
中央本级重大增减支项目(2060302-2101-11,2060302-2101-10) （2060302-2101-11,2060302-2101-10）
江西省教育厅科学技术研究项目(GJJ211269) （GJJ211269）
