中药材2024，Vol.47Issue(10)：2527-2534,8.DOI:10.13863/j.issn1001-4454.2024.10.021
基于低温研磨结合HS-SPME-GC-MS测定竹叶榕不同部位挥发性成分
Analysis of Volatile Components in Different Parts of Ficus stenophylla Using Low-Temperature Grinding Combined with HS-SPME-GC-MS
摘要
Abstract
Objective:To investigate the variations in volatile components among different parts of Ficus stenophylla.Methods:After low-temperature grinding treatment,samples from various parts of Ficus stenophylla were subjected to headspace solid-phase microex-traction gas chromatography mass spectrometry(HS-SPME-GC-MS)for the extraction and analysis of their volatile components.Qualita-tive identification of these volatile components was performed using similarity searches and retention index methods.Furthermore,rela-tive content of each component was calculated using peak area normalization.Lastly,differences in the composition of different parts were investigated using clustering heatmaps,principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA).Results:A total of 104 volatile components were detected across various parts of Ficus stenophylla,with 60,20,36 and 42 volatile components detected in the adventitious roots,primary roots,stems and leaves,respectively.Eight common volatile components were i-dentified among these four parts,predominantly comprising terpenoids.Cluster heatmap analysis revealed that stems and primary roots clustered together,while leaves and adventitious roots formed distinct clusters.PLS-DA analysis identified 28 components as key differ-entiating factors for distinguishing between different parts of Ficus stenophylla.Conclusion:Differences in the types and quantities of vol-atile components are observed across various parts of Ficus stenophylla.The study provides robust evidence and support for the in-depth exploration and utilization of Ficus stenophylla resources,while also opening up new possibilities for research and applications in related fields.关键词
竹叶榕/HS-SPME-GC-MS/挥发性成分/保留指数/聚类热图/PCA/PLS-DAKey words
Ficus stenophylla Hemsl./HS-SPME-GC-MS/Volatile components/Retention index/Cluster heatmap/PCA/PLS-DA分类
医药卫生引用本文复制引用
汤邝杰,曾雷,段金龙,王志宏,徐巧林,刘小华,郑奕雄..基于低温研磨结合HS-SPME-GC-MS测定竹叶榕不同部位挥发性成分[J].中药材,2024,47(10):2527-2534,8.基金项目
广东省林业科技创新项目(2021KJCX004) （2021KJCX004）
