中药材2024，Vol.47Issue(10)：2535-2541,7.DOI:10.13863/j.issn1001-4454.2024.10.022
沙苑子饮片的指纹图谱建立及7个黄酮类成分含量测定
Establishment of Fingerprint and Determination of Seven Flavonoid Components in Astragali Complanati Semen Decoction Pieces
摘要
Abstract
Objective:To establish the HPLC fingerprint and multi-component content determination method for the decoction pieces of Astragali Complanati Semen,and to evaluate the quality of Astragali Complanati Semen by combining with the chemical pattern recognition method.Methods:The fingerprint of 22 batches of Astragali Complanati Semen decoction pieces was established by HPLC gradient elution,and the similarity evaluation was carried out by using the"Similarity Evaluation System of Traditimal Chinese Medicine Chromatographic Fingerprint(2012 Edition)".Cluster analysis,principal component analysis and orthogonal partial least squares-dis-criminant analysis were performed using SPSS 23.0 and SIMCA 14.1 software to screen the differential components.The contents of seven flavonoid components were determined.Results:The fingerprint of 22 batches of Astragali Complanati Semen decoction pieces was labeled with 16 peaks,and the similarity was greater than 0.950.The samples could be classified into 4 groups according to the origin by cluster analysis.Nine differential markers were obtained by synthetic analysis.The contents of ampelopsin,astragalin,complanatoside B,complanatoside,quercetin,kaempferol and formononetin in the samples ranged from 2.2699～3.1909 mg/g,0.1473～0.2576 mg/g,0.1730～0.7083 mg/g,0.5028～0.7486 mg/g,0.0448～0.2523 mg/g,0.0266～0.0441 mg/g,0.0588～0.2380 mg/g,respectively.Conclusion:The HPLC fingerprint and multi-component content determination method established in this study has good stability and feasibility,and can provide a reference for the quality evaluation of Astragali Complanati Semen decoction pieces by combining with the chemical pattern recognition.关键词
沙苑子/HPLC/指纹图谱/黄酮/化学模式识别/含量测定Key words
Astragali Complanati Semen/HPLC/Fingerprint/Flavonoids/Chemical pattern recognition/Content determination分类
医药卫生引用本文复制引用
刘梦桐,袁诗农,程浩,宋军娜,段绪红..沙苑子饮片的指纹图谱建立及7个黄酮类成分含量测定[J].中药材,2024,47(10):2535-2541,7.基金项目
河北省中医药管理局科研计划项目(2024096) （2024096）
河北中医药大学优秀创新团队项目(YXTD2023007) （YXTD2023007）
