中药材2024，Vol.47Issue(10)：2578-2583,6.DOI:10.13863/j.issn1001-4454.2024.10.029
滇重楼皂苷通过PI3K/AKT通路调控内质网应激介导细胞凋亡发挥抗肝癌作用的机制研究
Study on the Mechanism of Anti-Liver Cancer Effect of Paridis Rhizoma Saponins by Regulating Endoplasmic Reticulum Stress-Mediated Apoptosis Through PI3K/AKT Pathway
摘要
Abstract
Objective:To observe the inhibitory effect of Paridis Rhizoma saponins on HepG2 cells,and to further study its anti-hepatoma mechanism.Methods:HepG2 cells were used to verify the action mechanism of drug,cell proliferation capacity was detected by MTT,cell migration capacity was detected by nicks and Transwell tests,cell morphological changes were observed by inverted micro-scope,cell proliferation capacity was detected by colony formation assay,cell apoptosis was detected by flow cytometry,ROS production level was detected by fluorescence method.The expressions of apoptosis-related proteins and pathway proteins were detected by Western Blot.Results:Paridis Rhizoma saponins could decrease proliferation,clonogenesis and migration of HepG2 cells,increase apoptosis rate and ROS level,decrease the expressions of Bcl-2,PI3K,p-PI3K,AKT,p-AKT,JAK2,STAT3,p-STAT3,mTOR and p-mTOR proteins,increase the expressions of Bax,cleaved Caspase-3,PERK,ATF4 and CHOP proteins.Conclusion:Paridis Rhizoma saponins plays an anti-liver cancer role by inhibiting PI3K/AKT pathway and activating PERK/ATF4/CHOP mediated apoptosis.关键词
滇重楼皂苷/抗肝癌/内质网应激凋亡/PI3K/AKT通路/PERK/ATF4/CHOP通路Key words
Paridis Rhizoma saponins/Anti-hepatoma/Endoplasmic reticulum stress-related apoptosis/PI3K/AKT pathway/PERK/ATF4/CHOP pathway分类
中医学引用本文复制引用
郭春香,赵微,陈文慧,冯晓异..滇重楼皂苷通过PI3K/AKT通路调控内质网应激介导细胞凋亡发挥抗肝癌作用的机制研究[J].中药材,2024,47(10):2578-2583,6.基金项目
国家自然科学基金项目(82060737,82160898) （82060737,82160898）
云南省科技厅科技计划项目(202301AT070253) （202301AT070253）
云南省科技厅中医联合专项项目(202101AZ070001-030,02101AZ070001-264) （202101AZ070001-030,02101AZ070001-264）
云南省科技人才与平台计划项目(202305AC060041) （202305AC060041）
云南省可持续利用研究重点实验室2023年度开放课题(202105AG070012XS23002) （202105AG070012XS23002）
