中南林业科技大学学报2025，Vol.45Issue(4)：98-107,10.DOI:10.14067/j.cnki.1673-923x.2025.04.009
不同砧木对5年生芽苗砧嫁接油茶叶片解剖结构及其生理特性的影响
Effects of rootstocks on anatomical structure and physiological characteristics of 5-year-old Camellia oleifera leaves grafted on bud seedlings
摘要
Abstract
[Objective]To explore the effects of rootstocks on leaf structure and physiology of grafted Camellia oleifera at the early fruit stage,reveal the law of rootstocks on the aboveground parts,and analyze the relationship between leaf structure and physiology,the study laid a theoretical foundation for the selection of rootstock for Camellia oleifera bud seedling grafting.[Method]'GW2''GS83-1'and'G8'were used as rootstocks for'GW2'panicle grafting to develop different rootstock combinations with the same panicle,the anatomical structure,resistance physiology,photosynthetic characteristics and chlorophyll fluorescence characteristics of the grafted plants were investigated at the age of 5 years,the differences of different stock combinations and the relationship between leaf structure and physiology of grafted Camellia oleifera were analyzed.[Result]1)There were significant differences in leaf anatomical structure among different stock combinations with the same panicle,the'GW2'rootstock combination had significantly higher leaf thickness(330.15 μm),palisade tissue thickness(180.97 μm)and sponge tissue thickness(149.19 μm)than other combinations,and lowest gate/sea ratio(1.22),the content of chlorophyll(12.61 mg·g-1)was significantly lower than that of other combinations;2)the effect of rootstock varieties on soluble protein content of 5-year-old grafted leaves was significant,but the effect on soluble sugar content was not significant.The soluble protein content of'G8'was also the highest(3.51 mg·g-1),and'G8'as rootstock was the highest(1.28)in palisade/sea ratio,indicating that'G8'had the most remarkable drought resistance;3)Rootstock had no significant effect on the activities of four antioxidant enzymes(SOD,POD,CAT,MDA),which may be due to the fact that the sampling time was in the less stress 5 months,and the antioxidant enzymes had not started the protective procedure.4)There were no significant effects on Fo,Fm,Fv/Fm,qP,qN of leaves,but significant effects on intercellular CO2 concentration,stomatal conductance and Y(II)value and ETR value of chlorophyll fluorescence characteristics of leaves,the actual photochemical quantum efficiency and apparent electron transfer efficiency of photosystem II of'GW2'rootstock combination are both at a high level,which may be due to the high affinity of the rootstock combination.[Conclusion]There are differences in leaf structure and physiological characteristics between five-year-old and five-year-old grafted plants on different rootstocks with the same panicle,'G8'as rootstock had the highest tissue thickness and soluble protein content in palisade/sponge,and had stronger drought resistance.'GW2'rootstock had significantly lower chlorophyll content than other combinations,the gate/sea ratio was positively correlated with chlorophyll,and the soluble sugar content was the highest.The actual photochemical quantum efficiency and apparent electron transfer efficiency of photosystem II were stronger,and the adaptability was stronger.关键词
油茶/嫁接/砧木/叶片解剖结构/生理特性Key words
Camellia oleifera/grafting/rootstock/leaf anatomy/physiological characteristics分类
农业科技引用本文复制引用
任可为,胡冬南,刘娟,邹中华,陶文杰,左静,周伟华,刘耀辉,陈豪,王毓曼..不同砧木对5年生芽苗砧嫁接油茶叶片解剖结构及其生理特性的影响[J].中南林业科技大学学报,2025,45(4):98-107,10.基金项目
国家自然科学基金项目(32060362) （32060362）
江西省研究生创新专项(YC2023-S386). （YC2023-S386）
