中药材2024，Vol.47Issue(12)：3065-3070,6.DOI:10.13863/j.issn1001-4454.2024.12.021
基于多成分定量及化学计量学分析的大戟属3种根类药材差异性研究
Study on the Differences of Three Common Root Medicinal Materials of Euphorbia Based on Multi-Component Quantitative and Chemometric Analysis
摘要
Abstract
Objective:To analyze the differences of the contents of gallic acid,corilagin,geraniin and ellagic acid in the roots of Euphorbia pekinensis,Euphorbia ebracteolata and Euphorbia kansui.Methods:Using the Ultimate·AQ-C18(250 mm×4.6 mm,5 μm)column,the mobile phase was acetonitrile-0.5%phosphoric acid with gradient elution.The flow rate was 1.0 mL/min,detection wave-length was 254 nm,column temperature was 35℃,injection volume was 10 μL.Results:The linear relationship of the above four com-ponents was good(r≥0.9989)in a certain range.The average recoveries were 95.00%to 106.37%.The RSD of precision,stability and repeatability tests was less than 5.00%.The results of stoichiometric analysis showed that geraniin and ellagic acid were the differ-ent components of the roots of Euphorbia pekinensis,Euphorbia ebracteolata and Euphorbia kansui,corilagin and ellagic acid were the different components of the roots of Euphorbia ebracteolata and Euphorbia kansui.Conclusion:A simple,feasible multi-index content de-termination method for three medicinal materials form Euphorbia is established,which provides a reference for the rational research and utilization of tannins in Euphorbia.关键词
京大戟/狼毒/甘遂/化学成分/化学计量学分析Key words
Roots of Euphorbia pekinensis Rupr./Roots of Euphorbia ebracteolata Hayata/Roots of Euphorbia kansui T.N.Liou ex T.P.Wang/Chemical compositions/Stoichiometric analysis分类
医药卫生引用本文复制引用
岑如愿,纪瑞锋,班建平,程感桃,何新..基于多成分定量及化学计量学分析的大戟属3种根类药材差异性研究[J].中药材,2024,47(12):3065-3070,6.基金项目
云浮市2022年中医药(南药)创新团队项目(云科函[2022]71号) （南药）
