中药材2024，Vol.47Issue(11)：2719-2723,5.DOI:10.13863/j.issn1001-4454.2024.11.008
甘松倍半萜合成酶基因的克隆及表达分析
Cloning and Expression Analysis of Sesquiterpene Synthetase Genes from Nardostachys jatamansi
摘要
Abstract
Objective:To screen the candidate genes of sesquiterpene synthetase from Nardostachys jatamansi,to clone and analyze their expression,so as to provide evidence for the molecular mechanism of quality formation of Nardostachys jatamansi.Methods:The primers were designed based on the transcriptome sequencing information and cloned from the cDNA library byusing PCR,and bioinfor-matics analysis of the candidate sesquiterpene synthetase genes was performed byusing online software.The differential expressions of candidate genes in different parts of the tissues of Nardostachys jatamansi were detected by real-time fluorescent quantitative PCR.Re-sults:Seven candidate sesquiterpene synthetase genes(named NjTPS1,NjTPS2 and NJTPS4-8)were cloned,all of which contained the structure of the key terpene skeleton-forming enzyme(TPS)gene family.The results the phylogenetic analysis of the family of 7 cloned genes and terpenoid synthetase genes of model plant Arabidopsis thaliana showed that TPS1,TPS2,TPS4 and TPS5 were closely related to Arabidopsis thaliana genes.In real-time quantitative PCR detection,the expressions of TPS1,TPS2,TPS4 and TPS7 were the highest in the root,but relatively low in other tissues(P<0.05).The expression of TPS5 in roots was the highest(P<0.01),but not in stems,leaves and flowers.The expressions of TPS6 and TPS8 were the highest in stems and lower in other tissues.Conclusion:The information and expression characteristics of 7 genes related to sesquiterpene synthesis of Nardostachys jatamansi are obtained in this study,which lays a foundation for further research on the regulatory mechanism of sesquiterpene synthetase of Nardostachys jatamansi.关键词
甘松/倍半萜合酶/转录因子/基因表达/生物信息学Key words
Nardostachys jatamansi DC./Sesquiterpene synthetase/Transcription factors/Gene expression/Bioinformatics分类
中医学引用本文复制引用
郝志禹,唐小慧,胡月莹,周妍汛,彭燕妮,裴瑾,陈江..甘松倍半萜合成酶基因的克隆及表达分析[J].中药材,2024,47(11):2719-2723,5.基金项目
国家自然科学基金项目(82274039) （82274039）
四川省自然科学基金项目(2023NSFSC0660) （2023NSFSC0660）
四川省"十四五"农作物及畜禽育种攻关项目(2021YFYZ0012-5) （2021YFYZ0012-5）
云南省科技计划项目(202304BI090020) （202304BI090020）
国家中医药多学科交叉创新团队项目(ZYYCXTD-D-202209) （ZYYCXTD-D-202209）
