中药材2024，Vol.47Issue(11)：2724-2729,6.DOI:10.13863/j.issn1001-4454.2024.11.009
蝉蜕配方颗粒的位点特异性PCR鉴别
Site-Specific PCR Identification of Cicadae Periostracum Formulation Granules
摘要
Abstract
Objective:To establish a particle-specific polymerase chain reaction(PCR)method for the identification of Cicadae Periostracum formulation granules,to effectively realize the identification of Cicadae Periostracum inmedicinal materials and different dosage forms,so as to ensure the safety and effectiveness of clinical medication.Methods:Based on the Cryptotympana pustulata cyto-chrome oxidaseⅠ(COⅠ)gene sequence,single nucleotide polymorphism(SNP)sites were screened,specific primers CT-F and CT-R were designed,and a specific PCR method was established for the identification of Cicadae Periostracum formulation granules.The main conditions affecting PCR reaction were optimized,and the durability and applicability of the method were investigated.Results:When the annealing temperature was 57℃and the number of cycles was 37 times,both the Cicadae Periostracum and its formulation granules showed a single bright band at 173 bp,while the counterfeit had no band.Conclusion:The established specific PCR identification meth-od can accurately identify Cicadae Periostracum,standard decoction freeze-dried powder and formulation granules,and also provide a reference for the quality standard research of Cicadae Periostracum formulation granules.关键词
蝉蜕/配方颗粒/聚合酶链式反应/单核苷酸多态性/分子鉴定Key words
Cicadae Periostracum/Formulation granules/Polymerase chain reaction/Single nucleotide polymorphism/Molecular i-dentification分类
中医学引用本文复制引用
范耀耀,谭斯尹,宋叶,刘闪闪,李国卫,潘礼业,罗宇琴..蝉蜕配方颗粒的位点特异性PCR鉴别[J].中药材,2024,47(11):2724-2729,6.基金项目
国家工信部2022年产业技术基础公共服务平台项目(2022230221) （2022230221）
2022年佛山市南海区重点领域科技攻关专项(南科[2023]20号-18) （南科[2023]20号-18）
