中药材2024，Vol.47Issue(12)：3097-3102,6.DOI:10.13863/j.issn1001-4454.2024.12.028
基于TNF-α/TNFR1/TRADD信号通路探讨定痫丸对癫痫小鼠海马神经元凋亡的影响
Effect of Dingxian Pills on Hippocampal Neuron Apoptosis in Epileptic Mice Based on TNF-α/TNFR1/TRADD Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of Dingxian Pills on hippocampal neuron apoptosis in epileptic mice based on TNF-α/TNFR1/TRADD pathway.Methods:60 Kunming mice were randomly divided into normal control group,model group,carbamazepine(30 mg/kg)positive control group and Dingxian Pills low(4.08 g/kg),medium(8.16 g/kg)and high(16.32 g/kg)dose groups with 10 in each group.The epileptic mouse model was established by pilocarpine induction,and the mice were given continuous administra-tion of the drug for 2 weeks,once a day,and the success of the model was observed by eleetroencephalogram.The pathological changes of hippocampus were observed by HE staining,hippocampal neuron apoptosis was observed by TUNEL staining,the expressions of TNF-α,TNFR1 and TRADD mRNA and proteins in hippocampus were detected by immunohistochemical method(IHC),Real-time PCR,Western Blot.Results:Compared with the normal control group,eleetroencephalogram continuous epileptic radio wave in model group was increased,hippocampal neuron morphological structure was damaged,hippocampal neuron apoptosis was aggravated,and TNF-α,TNFR1,TRADD mRNA and protein expressions in hippocampus were significantly increased(P<0.05).Compared with model group,eleetroencephalogram epileptic discharge was reduced,hippocampal neuron morphological structure was significantly recovered,hipp-ocampal neuron apoptosis was improved to varying degrees,and the expressions of TNF-α,TNFR1 and TRADD mRNA and protein in hippocampus were significantly decreased in each administration group(P<0.05).Conclusion:Dingxian Pills can alleviate the apoptosis of hippocampal neurons by inhibiting TNF-α/TNFR1/TRADD signaling pathway,and finally play an anti-epileptic role.关键词
定痫丸/癫痫/海马/神经元凋亡/TNF-α/TNFR1/TRADD信号通路Key words
Dingxian Pills/Epilepsy/Hippocampus/Neuron apoptosis/TNF-α/TNFR1/TRADD Signaling pathway分类
医药卫生引用本文复制引用
王钦,刘金文,范晶晶,曾佳威,谭雅匀,蔡伦,刁丽梅..基于TNF-α/TNFR1/TRADD信号通路探讨定痫丸对癫痫小鼠海马神经元凋亡的影响[J].中药材,2024,47(12):3097-3102,6.基金项目
国家自然科学基金项目(82360875,81960858) （82360875,81960858）
广西科技基地和人才专项项目(桂科AD22035168) （桂科AD22035168）
广西中医药大学研究生教育创新计划项目(YCBXJ2023005) （YCBXJ2023005）
