中药材2024，Vol.47Issue(12)：3103-3107,5.DOI:10.13863/j.issn1001-4454.2024.12.029
双氢青蒿素通过调节SLC7A11/GPX4信号通路诱导卵巢癌细胞铁死亡
Study on the Effect of Dihydroartemisinin on Ferroptosis in Ovarian Cancer Cells by Regulating SLC7A11/GPX4 Signaling Pathway
摘要
Abstract
Objective:To investigate the effect and mechanism of dihydroartemisinin on ferroptosis in ovarian cancer SKOV3 cells.Methods:SKOV3 cells were treated with different concentrations of dihydroartemisinin,and the cell viability was detected by CCK-8;Cell migration ability was observed by cell scratch test.The levels of intracellular reactive oxygen species were detected by immunofluo-rescence.The levels of Fe2+and glutathione in cells were detected by the kit.Western Blotting and qPCR were used to detect the ex-pressions of ferroptosis related proteins and genes.Results:Dihydroartemisinin significantly decreased SKOV3 cell viability,migration a-bility and glutathione level,significantly increased ROS fluorescence intensity and Fe2+level in SKOV3 cells,and significantly decreased SLC7A11,GPX4 mRNA and protein expressions in SKOV3 cells(P<0.05 or P<0.01).The action of dihydroartemisinin was concentra-tion-dependent.Conclusion:Dihydroartemisinin can inhibit the proliferation of SKOV3 cells and induce ferroptosis by inhibiting SLC7A11/GPX4 signaling activation,thus playing an anti-ovarian cancer role.关键词
卵巢癌/双氢青蒿素/SLC7A11/GPX4轴/铁死亡/活性氧Key words
Ovarian cancer/Dihydroartemisinin/SLC7A11/GPX4 Axis/Ferroptosis/Reactive oxygen species分类
医药卫生引用本文复制引用
陈露,李由由,杲飞莹,邢悦,康璐瑶,卢丹..双氢青蒿素通过调节SLC7A11/GPX4信号通路诱导卵巢癌细胞铁死亡[J].中药材,2024,47(12):3103-3107,5.基金项目
国家自然科学基金面上项目(82072088) （82072088）
