中药材2024，Vol.47Issue(12)：2957-2965,9.DOI:10.13863/j.issn1001-4454.2024.12.003
林下栽培金线莲最适采收期研究
Study on the Optimal Harvesting Period of Anoectochilus roxburghii Cultivated Under Forest Conditions
摘要
Abstract
Objective:To investigate the fresh weight,dry weight and quality changes of Anoectochilus roxburghii cultivated under forest conditions in Fujian at different cultivation ages,so as to provide a comprehensive basis for the determination of the optimal har-vesting period.Methods:Monthly collections of Anoectochilus roxburghii samples cultivated under forest conditions for 0 to 9 months were performed,and the fresh weight,dry weight,water extraction rate,alcohol extraction rate,ash content and protein content were deter-mined.HPLC method was used to measure the contents of narcissoside,rutin,quercetin,quercitrin,kaempferol,isorhamnetin and kin-senoside.The ultraviolet method was used to determine the contents of crude polysaccharides,total flavonoids and total polyphenols.The antioxidant activities of Anoectochilus roxburghii extracts at different harvest times were compared using DPPH and FRAP methods.Cor-relation analysis and principal component analysis were used to explore the quality evaluation indicators and optimal harvest time.Re-sults:Anoectochilus roxburghii cultivated under forest conditions for 6 to 8 months had higher dry product yield.The uncultivated tissue culture seedlings and short-term cultivation under the forest showed higher kinsenoside content;The ash and protein content of unculti-vated tissue culture seedlings were the highest,and generally decreased during the cultivation process;The antioxidant activity of Anoec-tochilus roxburghii cultivated under forest conditions for 2,6 and 8 months was higher than that of 0 and 4 months.The planting month was significantly positively correlated with the drying rate,water extraction rate,crude polysaccharide content and narcissoside content.The top three comprehensive quality scores of Anoectochilus roxburghii were cultivated under the forest for 6,8 and 7 months,respective-ly.Conclusion:The optimal harvest time for Anoectochilus roxburghii cultivated under forest conditions is 6 to 8 months.关键词
金线莲/林下栽培/品质/采收期Key words
Anoectochilus roxburghii(Wall.)Lindl./Cultivated under forest conditions/Quality/Harvesting period分类
医药卫生引用本文复制引用
张武君,赵云青,刘保财,黄颖桢,蔡净蓉,陈菁瑛..林下栽培金线莲最适采收期研究[J].中药材,2024,47(12):2957-2965,9.基金项目
福建省科技计划公益类项目(2023R1105) （2023R1105）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021003-5) （XTCXGC2021003-5）
福建省农业科学院药用植物科技创新团队项目(CXTD2021014-2) （CXTD2021014-2）
