作物学报2025，Vol.51Issue(5)：1166-1177,12.DOI:10.3724/SP.J.1006.2025.44175
芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析
QTL mapping and candidate gene analysis of glucosinolate content in various tissues of Brassica juncea
摘要
Abstract
Glucosinolates are critical secondary metabolites that play a pivotal role in the growth and development of rapeseed,as well as in its defense against diseases and pests.In this study,a mapping population comprising 197 recombinant inbred lines(RILs)of Brassica juncea was utilized to investigate glucosinolate distribution and genetic regulation.The glucosinolate contents in leaves,stems,buds,and seeds were quantified across all lines.The results revealed significant differences in glucosinolate con-tent among different tissues within the same line and considerable variation across lines within the species,exhibiting an overall normal distribution.Correlation analysis showed a strong positive correlation in glucosinolate content among buds,stems,and seeds,as well as a notable positive correlation between leaves and flower buds.To further elucidate the genetic regulation of glu-cosinolate content,quantitative trait locus(QTL)mapping was conducted,resulting in the identification of 9,10,9,and 18 QTLs associated with glucosinolate content in leaves,stems,buds,and seeds,respectively.By integrating QTL interval sequence infor-mation with gene expression data,six candidate genes were preliminarily identified.Among them,BjuB018426(GTR1)and BjuB020498(GTR2)were implicated in the transport of glucosinolates from vegetative tissues to reproductive tissues,suggesting their potential roles as key regulatory genes underlying the differential distribution of glucosinolates observed in this study.These findings provide valuable insights into the mechanisms governing glucosinolate synthesis and distribution across various tissues in Brassica juncea.Moreover,they offer a foundational basis for breeding multifunctional varieties with tailored glucosinolate profiles to meet diverse agricultural and industrial needs.关键词
芥菜型油菜/硫苷含量/QTL定位/候选基因/硫苷转运Key words
Brassica juncea/glucosinolate contents/QTL mapping/candidate genes/glucosinolate transport引用本文复制引用
张金泽,周庆国,肖莉晶,金海润,欧阳青静,龙旭,晏中彬,田恩堂..芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J].作物学报,2025,51(5):1166-1177,12.基金项目
本研究由贵州省科技支撑计划项目(黔科合支撑[2022]重点031),国家自然科学基金项目(32160483,32360497)和贵州大学国家自然科学基金后补助项目([2023]093号). This study was supported by the Guizhou Provincial Science and Technology Support Program(Guizhou Kehe Support[2022]Key 031),the National Natural Science Foundation of China(32160483,32360497),and the Post-Funded Project for National Natural Science Foundation of Guizhou University([2023]No.093). （黔科合支撑[2022]重点031）
