作物学报2025，Vol.51Issue(5)：1189-1197,9.DOI:10.3724/SP.J.1006.2025.44062
甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究
Correlation between salicylic acid and anthocyanins accumulation in seeds of different varieties in Brassica napus
摘要
Abstract
The color difference between yellow-and black-seeded Brassica napus is significant,with proanthocyanidins being one of the main factors influencing this variation.To explore the regulatory role of the plant hormone salicylic acid(SA)in rape-seed seed color,this study conducted in-depth analyses of anthocyanins and SA at different developmental stages in seeds and seed coats of B.napus varieties,including the black-seeded"Zhongshuang11"(ZS11)and the yellow-seeded"Huang'aizao"(HAZ),"Huahuang1"(HH1),and GH06.Observations of seed sections and ultra-high performance liquid chromatogra-phy-tandem mass spectrometry(UHPLC-MS/MS)were employed.The results showed that black-seeded ZS11 exhibited signifi-cant pigment accumulation at 25 days after flowering(DAF),whereas yellow-seeded HAZ lacked a pigment-accumulating layer.From 20 DAF,anthocyanins A1 and B2 in the seeds and seed coats of all four varieties gradually increased,peaking at 25 DAF.In ZS11,anthocyanin A1 and B2 levels remained stable and high after 25 DAF,and were significantly higher than those in the three yellow-seeded varieties(HAZ,GH06,and HH1)at the 0.01 significance level.SA content also gradually increased after 15 DAF,peaking between 20 and 25 DAF,and subsequently decreased.Notably,after 20 DAF,SA concentrations in the seeds and seed coats of ZS11 were significantly higher than those in the yellow-seeded varieties.Further analysis revealed a positive correlation between SA concentration and anthocyanin accumulation in B.napus.Moreover,exogenous application of SA(1 mmol L-1)sig-nificantly increased pigment accumulation in the seed coats of HAZ and resulted in a higher percentage of dark brown seeds.These findings indicate that elevated SA levels promote anthocyanin accumulation in B.napus seeds and seed coats,contributing to the formation of black seeds,while lower SA levels favor the development of yellow seed traits.This study provides new in-sights into the role of SA in seed color regulation and offers a theoretical basis for breeding yellow-seeded B.napus varieties.关键词
甘蓝型油菜/种子颜色/水杨酸/原花色素Key words
Brassica napus/seed color/salicylic acid/proanthocyanins引用本文复制引用
夏琦,郭滢,王坤美,王思忆,巨建业,彭雅雯,刘忠松,夏石头..甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究[J].作物学报,2025,51(5):1189-1197,9.基金项目
本研究由国家自然科学基金项目"甘蓝型油菜黄籽性状遗传稳定性的调控机制研究"(U20A2029)和国家自然科学基金项目"油菜GYF1与互作因子对真菌病害抗性的调控机理研究"(31971836)资助. This study was supported by the National Natural Science Foundation of China"Regulatory Mechanism of Yellow Seed Genetic Stability in Brassica napus"(U20A2029)and the National Natural Science Foundation of China"Studies on Regulatory Mechanism of Canola GYF1 Protein and its Interaction Factors on Resistance to Fungal Diseases"(31971836). （U20A2029）
