大麦PRX基因家族全基因组鉴定及其干旱胁迫下的表达分析
Genome-wide identification of PRX gene family and analysis of their expres-sions under drought stress in barley
摘要
Abstract
The Class Ⅲ peroxidase(PRX)gene family plays a crucial role in regulating plant growth,development,and responses to abiotic stress.Barley(Hordeum vulgare L.),a typical C3 plant,has been relatively underexplored regarding the functional characterization of its HvPRX gene family.In this study,we performed a comprehensive analysis of HvPRX genes using bioin-formatics tools and investigated their expression patterns under drought stress induced by 20％PEG-6000 treatment.A total of 178 HvPRX gene family members were identified in the barley genome and were named HvPRX1-HvPRX178 based on their chromosomal positions.Phylogenetic analysis grouped the peroxidases of barley,rice,and Arabidopsis into five subfamilies,in-dicating evolutionary conservation.Gene structure and domain analyses revealed high conservation within the same subfamilies.Gene duplication analysis showed that 15 HvPRX genes(8％)underwent segmental duplication,while 34 HvPRX genes(67％)arose from tandem duplication,highlighting the critical role of tandem duplication events in HvPRX gene expansion.Interspecies collinearity analysis between barley and Arabidopsis identified four direct orthologous PRX gene pairs,suggesting that large-scale molecular evolution events occurred during the divergence from monocotyledons to dicotyledons.Transcriptome analysis demon-strated that HvPRX gene expression patterns varied between barley roots and leaves.Promoter analysis revealed that 99 HvPRX genes contained cis-acting elements associated with drought stress responses.Finally,qRT-PCR analysis was used to validate the expression profiles of HvPRX genes under drought stress.The expression levels of HvPRX1,HvPRX18,HvPRX63,HvPRX160,and HvPRX167 were significantly upregulated three hours after treatment with 20％PEG-6000.These findings provide valuable insights into the biological functions and molecular mechanisms of HvPRX genes in barley's drought resistance.This study also lays a foundation for breeding stress-tolerant crop varieties.关键词
大麦/PRX基因家族/生信分析/干旱胁迫Key words
barley/PRX gene family/bioinformatics analysis/drought stress引用本文复制引用
陆雯佳,王化俊,汪军成,姚立蓉,张宏,司二静,杨轲,孟亚雄,李葆春,马小乐..大麦PRX基因家族全基因组鉴定及其干旱胁迫下的表达分析[J].作物学报,2025,51(5):1198-1214,中插3-中插7,22.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05-02A-02),东西部科技协作专项(25CXNA030),国家重点实验室开放基金项目(GSCS-2021-02),甘肃省现代农业产业技术体系(2025),甘肃农业大学公招博士科研启动项目(GAU-KYQD-2022-10),甘肃省教育厅产业支撑计划项目(2021CYZC-12),甘肃农业大学伏羲青年英才计划(GAUfx-04Y011,Gaufx-03Y06),甘肃省陇原青年英才项目(2023)资助. This study was supported by the China Agriculture Research System of MOF and MARA(CARS-05-02A-02),the East West Science and Technology Cooperation Special Project(25CXNA030),the State Key Laboratory of Aridland Crop Science Open Fund(GSCS-2021-02),the Modern Agricultural Industry Technology System in Gansu Province(2025),the Scientific Research Start-up Funds for Openly-recruited Doctors of Gansu Agricultural University(GAU-KYQD-2022-10),the Industrial Support Project of Colleges and Universities in Gansu Pro-vince(2021CYZC-12),the Fuxi Young Talents Fund of Gansu Agricultural University(GAUfx-04Y011,Gaufx-03Y06),and the Gansu Pro-vince Longyuan Youth Talent Project(2023). （CARS-05-02A-02）
