高、低Cd积累小麦对Cd胁迫的转录组学响应差异
Differences in transcriptomic responses to cadmium stress in high/low-Cd-accumulation wheat
摘要
Abstract
Cadmium(Cd)is readily absorbed by wheat,posing a significant threat to human health.However,the molecular mechanisms underlying wheat's response to Cd stress remain poorly understood.Investigating these mechanisms is essential for developing low-Cd-accumulating wheat varieties through genetic improvement.In this study,hydroponic culture combined with transcriptome sequencing was used to analyze gene regulatory network changes in two wheat varieties with differing Cd accumu-lation capacities(Jimai 22 and Zhoumai 32)under Cd stress at concentrations of 0,0.05 mmol L-1,and 0.10 mmol L-1.Functional enrichment analyses using the Kyoto Encyclopedia of Genes and Genomes(KEGG),Gene Ontology(GO),and Protein-Protein Interaction(PPI)networks revealed that Cd stress induced the expression of defense-related genes.The endoplasmic reticulum protein processing pathway was the most significantly enriched upregulated pathway in Jimai 22 under 0.05 mmol L-1 Cd stress,while the benzoxazinoid biosynthesis pathway was highly enriched in Jimai 22 under 0.10 mmol L-1 Cd stress.Additionally,the ribosomal protein uL13 family was identified as a central hub in the PPI network under Cd stress,highlighting its importance in maintaining ribosomal function.Key transporters,including TaNRAMP1,TaNRAMP2,TaNRAMP5,TaZIP6,and TaABCG36,were found to play pivotal roles in Cd uptake and accumulation.Moreover,transcription factors such as WRKY,MYB,bHLH,and bZIP were upregulated under Cd stress,contributing to the alleviation of Cd-induced damage.Weighted gene co-expression network analysis(WGCNA)identified LOC123168319 and LOC123145825 as potential candidate genes associated with Cd ac-cumulation.The differentially expressed genes and metabolic pathways identified in this study provide valuable resources for genetic improvement of wheat.Technologies such as CRISPR/Cas9 can be employed to reduce Cd absorption and accumulation,offering insights into wheat's Cd resistance mechanisms and supporting the breeding of low-Cd wheat varieties.关键词
Cd胁迫/小麦/差异表达基因/蛋白-蛋白相互作用/加权基因共表达网络Key words
Cd stress/wheat/differentially expressed gene/protein-protein interaction/weighted gene co-expression network引用本文复制引用
王青,王伊秀,李越男,吕永辉,张海波,刘娜,程红艳..高、低Cd积累小麦对Cd胁迫的转录组学响应差异[J].作物学报,2025,51(5):1230-1247,18.基金项目
本研究由国家自然科学基金项目(42107044),山西农业大学杰青优青培育工程项目(2022YQPYGC07),来晋工作奖励基金(SXBYKY2021038),山西省科技重大专项计划"揭榜挂帅"项目"特优食用菌产业关键技术研究与应用示范"(202301140601015)和山西农业大学"特""优"高质量发展科技支撑工程项目(TYGC24-03)资助. This study was supported by the National Natural Science Foundation of China(42107044),the Distinguished and Excellent Young Scholar Cultivation Project of Shanxi Agricultural University(2022YQPYGC07),the Shanxi Province Excellent Doctor Award Fund(SXBYKY2021038),the Shanxi Province Major Science and Technology Special Plan"Rank-Listed Project"titled"Research and Applica-tion Demonstration of Key Technologies in the Super Quality Edible Mushroom Industry"(202301140601015),and the Shanxi Agricultural University"Special"and"Excellent"Agricultural High Quality Development Science and Technology Support Project(TYGC24-03). （42107044）
