中国奶牛Issue(4)：22-26,5.DOI:10.19305/j.cnki.11-3009/s.2025.04.005
微滴式数字PCR在兽医学检测中的应用进展
Progress in the Application of Droplet Digital PCR in Veterinary Detection
摘要
Abstract
Droplet digital PCR has become an important tool for veterinary molecular biology research by dividing the reaction system into a large number of independent droplets.Each droplet contains a single or a few nucleic acid molecules to achieve absolute quantitative detection.It has the advantages of high sensitivity,high specificity and no standard curve.It is widely used in pathogen detection,vaccine development,drug resistance analysis,gene expression and microbial community analysis.On this basis,this paper reviewed the principle and advantages of digital droplet PCR,and its application and prospects in veterinary detection,to provide a reference for related research.关键词
微滴式数字PCR/核酸检测/绝对定量Key words
Droplet digital PCR/Nucleic acid testing/Absolute quantification分类
农业科技引用本文复制引用
杨祖丽,胡长敏..微滴式数字PCR在兽医学检测中的应用进展[J].中国奶牛,2025,(4):22-26,5.
