中药材2024，Vol.47Issue(6)：1343-1350,8.DOI:10.13863/j.issn1001-4454.2024.06.002
人参PgSL基因的克隆及表达分析
Cloning and Expression Analysis of PgSL Gene from Panax ginseng
摘要
Abstract
Objective:To clone the strigolactone esterase gene PgSL from Panax ginseng,conduct bioinformatics analysis,and de-termine the expression level of PgSL gene under nitrogen deficiency,phosphorus deficiency,and treatment with the synthetic strigolac-tone analog GR24.Methods:Based on the CDS sequence of Panax ginseng PgSL gene obtained by high-throughput sequencing,the CDS sequence of PgSL gene was amplified by homologous recombination and golden gate seamless cloning method,and the encoded amino acid sequence was submitted to NCBI,Protparam,SOPMA and other websites or software for bioinformation analysis.Real-time fluores-cent quantitative PCR(qRT-PCR)was used to detect the expression level of PgSL gene in root tissues of Panax ginseng treated with ni-trogen deficiency,phosphorus deficiency and exogenous GR24.Results:The CDS sequence of Panax ginseng PgSL gene was 816 bp,en-coding 271 amino acids.PgSL protein belonged to MhpC protein,which was relatively stable and belonged to hydrophobic protein,α he-lix was the main component.There was a close phylogenetic relationship between PgSL protein and strigolactone esterase in Heracleum sosnowskyi,Juglans regia and Gossypium arboreum.In comparison to the control group,upregulation of the PgSL gene was observed in response to nitrogen deficiency treatment,whereas its expression decreased upon application of exogenous GR24.Conversely,no notable alteration was detected in the expression of the PgSL gene following phosphorus deficiency treatment,however,an increase in PgSL gene expression was noted following exogenous GR24 treatment.Moreover,under conditions of nitrogen and phosphorus deficiency treatment,a decrease in PgSL gene expression was observed,which was subsequently reversed to an increase upon application of exogenous GR24.Conclusion:The CDS sequence and expression information of PgSL gene are obtained by cloning,by cloning which can provide reference for the subsequent gene function study,and the regulation of nitrogen and phosphorus nutrition in Panax ginseng cultivation.关键词
人参/独脚金内酯/氮和磷/基因表达分析Key words
Panax ginseng C.A.Mey./Strigolactone/Nitrogen and phosphorus/Gene expression analysis分类
中医学引用本文复制引用
梁浩,孙海,邵财,周季欣,吕柏辰,朱家鹏,张亚玉..人参PgSL基因的克隆及表达分析[J].中药材,2024,47(6):1343-1350,8.基金项目
国家现代农业产业技术体系项目(CARS-21) （CARS-21）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-ISAPS) （CAAS-ASTIP-2021-ISAPS）
