中药材2024，Vol.47Issue(6)：1356-1360,5.DOI:10.13863/j.issn1001-4454.2024.06.004
不同生长时期灵芝子实体主要代谢产物含量及抗氧化活性比较分析
Comparative Analysis of Main Metabolites Contents and Antioxidant Activity of Ganoderma lucidum Fruiting Body at Different Growth Stages
摘要
Abstract
Objective:To explore the changes of main metabolites contents and antioxidant activity of Ganoderma lucidum fruiting body at different growth stages,so as to provide theoretical basis for the cultivation and harvesting of Ganoderma lucidum.Methods:Using Ganoderma lucidum strain CZ-3 as the test strain and bed-log as the cultivation medium,the changes of main agronomic traits,primary metabolites(polysaccharides,total protein,16 kinds of amino acids)contents,main secondary metabolites(triterpenes and ste-rols,ganodermic acid A,ergosterols)contents and antioxidant biological activities in fruiting body at six main growth stages of Ganoder-ma lucidum,including the primordium differentiation stage,stalk differentiation stage,cap differentiation stage,the cap maturity stage,the spore middle stage and the spore end stage unde covered soil cultivation pattern,were investigated.Results:From the cap maturity stage to the spore end stage,the main agronomic traits of Ganoderma lucidum fruiting body tended to be stable,and reached the highest in the growth period of Ganoderma lucidum,with no significant differences.During the growth period of Ganoderma lucidum,the polysac-charide content was increased firstly and then decreased,the highest content was 1.220%at the cap maturity stage,the contents of pro-tein,16 kinds of amino acids,triterpenes and sterols,ganodermic acid A and ergosterols showed a tendency of decreasing gradually with the growth of the fruiting body generally,and the contents were the highest at the primordium differentiation stage.There were differ-ences in antioxidant biological activities such as ABTS free radical scavenging ability,DPPH free radical scavenging ability and·OH free radical scavenging ability in fruiting body at different growth stages.The antioxidant activity of Ganoderma lucidum fruiting body was the strongest at the primordium formation stage,and decreased to different degrees with the extension of the growth stage.Conclu-sion:The main agronomic traits of Ganoderma lucidum fruiting body tend to be stable in the cap maturity stage,and the content of main metabolites and antioxidant activity shows a gradual decreasing trend with the the growth of Ganoderma lucidum.If the metabolites con-tent and antioxidant activity of Ganoderma lucidum fruitum body are uesd as targets,the differentiation stage of Ganoderma lucidum bud can be selected as the harvesting time.If the target is fruiting body biomass,the cap maturity stage can be selected as the harvesting time.关键词
灵芝/子实体/代谢产物/抗氧化活性Key words
Ganoderma lucidum(Leyss.ex Fr.)Karst./Fruiting body/Metabolites/Antioxidant activity分类
中医学引用本文复制引用
李学龙,刘国丽,高英旭,李宏亮,张鹏,李超..不同生长时期灵芝子实体主要代谢产物含量及抗氧化活性比较分析[J].中药材,2024,47(6):1356-1360,5.基金项目
辽宁省农业科学院院长基金项目(2023MS0502) （2023MS0502）
辽宁省农业科学院横向联合项目(2023210105001004) （2023210105001004）
辽宁省农业科学院食药用菌栽培学科项目(2019DD072311) （2019DD072311）
