中国渔业质量与标准2024，Vol.14Issue(6)：1-9,9.DOI:10.3969/j.issn.2095-1833.2024.06.001
超高效液相色谱质谱法同时测定水产品中58种药物残留
Simultaneous determination of 58 drug residues in aquatic products by UPLC-MS/MS
吴丹 1林英扬 1张杨 1肖曼 1魏芝芝 1张微 1徐媛原 1李凯华 1张兵1
作者信息
- 1. 深圳市质量安全检验检测研究院,广东 深圳 518055
- 折叠
摘要
Abstract
An ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS/MS)method was es-tablished for the simultaneous detection of 58 drug residues across 8 categories in aquatic products,including quinolo-nes,benzodiazepines,β-receptor agonist,nitroimidazoles,benzimidazoles,glucocorticoids,nonsteroidal anti-in-flammatory drugs,and sulfonylureas.The samples were extracted by 80％acetonitrile aqueous solution(containing 0.2％formic acid)and purified using a Waters PRiME HLB solid-phase extraction column.The analytes were quantified by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)with a matrix-matched standard curve.The correlation coefficients for all 58 veterinary drugs were greater than 0.99.At three spiked concentration levels,the relative standard deviations ranged from 1.9％to 14.7％,and the average recovery rates were between 78.9％and 112.8％.The method was applied to 30 commercially available aquatic samples,where quinolones were detected in 9 samples at levels ranging from 2.23 to 425.00 μg/kg.The method is simple,fast,and suitable for the simultaneous detection and screening of multiple drug residues in aquatic products.It also serves as a reference for the development of high-throughput methods for the safety monitoring of aquatic products.[Chinese Fishery Quality and Standards,2024,14(6):01-09]关键词
高效液相色谱—串联质谱法/水产品/药物残留Key words
UPLC-MS/MS/aquatic product/drug residues分类
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吴丹,林英扬,张杨,肖曼,魏芝芝,张微,徐媛原,李凯华,张兵..超高效液相色谱质谱法同时测定水产品中58种药物残留[J].中国渔业质量与标准,2024,14(6):1-9,9.
