Abstract
Objective:To study the chemical constituents from the flowers of Camellia ptilosperma and their cytotoxicity in vitro and α-glucosidase inhibitory properties.Methods:Modern separation and purification techniques were used to isolate the compounds from the ethanol extract of Camellia ptilosperma flowers.The structures of the isolated compounds were identified by physicochemical properties and nuclear magnetic resonance spectroscopy.The cytotoxicity and α-glycosidase inhibition of the compounds on six tumor cells were tested.Results:A total of 27 compounds were isolated from the 70%ethanol extract of Camellia ptilosperma flowers and identified as 7,3',4'-trihydroxyflavanone(1),7,3',5'-trihydroxyflavanone(2),catechin(3),(2R,3R)-3,5,6,7,8,3',4'-heptahydroxyflavanone(4),isorhamnetin-3-O-β-D-glucoside(5),isorhamnetin-3-O-neohesperidoside(6),quercetin-7-O-β-D-glucoside(7),quercetagetin-7-O-β-D-glucoside(8),isoquercitrin(9),quercetin-3'-O-β-D-glucoside(10),kaempferol-3-O-gentiobioside(11),3,5,7,4'-tetrahydroxyflavone-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(12),ononin(13),calycosin-7-O-β-D-glucopyranoside(14),pratensein-7-O-β-D-glucoside(15),schaftoside(16),ferulic acid(17),3,4-dihydroxycinnamic acid(18),β-hydroxypropiosyringone(19),3-methoxy-4,9-dihydroxypropiophenone(20),chlorogenic acid(21),neochlorogenic acid(22),cryptochlorogenic acid(23),protocatechuic acid(24),1-(2-ethylphenyl)ethane-1,2-ethanediol(25),pistillarin(26),22-O-angeloyl-3β,15α,16α,21α,28-pentahydroxyolean-12-en-3-O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)]-β-D-glucuronopyranoside(27),respec-tively.Conclusion:All of the compounds are isolated from Camellia ptilosperma for the first time.Compound 25 shows strong cytotoxicity to BEL-7402,compound 27 showos strong cytotoxicity to HepG2.Compounds 22 and 25 show strong inhibitory activity against 0.25 U/mL α-glycoside solution.关键词
毛籽金花茶/化学成分/细胞毒性/α-糖苷酶抑制活性Key words
Camellia ptilosperma S.Y Liang&Q.D.Chen/Chemical constituents/Cytotoxicity/Inhibition activity of α-glycosidase分类
医药卫生
