中国烟草科学2025，Vol.46Issue(2)：26-34,9.DOI:10.13496/j.issn.1007-5119.2025.02.004
烟草健康株与根结线虫发病株根际微生态差异分析
Comparative Analysis of Rhizosphere Microecology Between Healthy and Root-Knot Nematode-Infected Tobacco Plants
摘要
Abstract
In order to analyze the relationship between rhizosphere soil microecological factors and the occurrence of tobacco root-knot nematode disease,a typical tobacco field with tobacco root-knot nematode disease was selected in Aziying Township,Songming County,Kunming City,Yunnan Province.Rhizosphere soil samples were collected from both diseased and healthy tobacco plants.The number of second-instar larvae of root-knot nematodes,soil chemical properties,enzyme activities,and microbial community structure were analyzed and compared.Results showed that the root-knot nematode pathogen was identified as Meloidogyne incognita.Compared with the rhizosphere soil of healthy tobacco plants,the number of root-knot nematodes in the rhizosphere soil of diseased tobacco plants increased by 4.29 times(p<0.01).Soil acid phosphatase activity,pH,and available phosphorus content were reduced by 86.98%(p<0.05),0.33 units(p<0.01),45.53%(p<0.05),respectively.In contrast,sucrase activity increased by 57.36%(p<0.05).The microbial balance,bacterial diversity and richness decreased,and the fungal diversity increased but the richness decreased.The bacterial genus Sphingomonas were enriched in the rhizosphere soil of the diseased tobacco plants,and the fungal phylum Ascomycota were enriched in the rhizosphere of the healthy tobacco plants.The number of 2nd instar larvae of root-knot nematode was significantly positively correlated with sucrase activity and Sphingomonas,and significantly negatively correlated with pH,acid phosphatase,available phosphorus and Ascomycota.Low soil pH,acid phosphatase activity and available phosphorus content,and high sucrase activity may be important factors in inducing tobacco root-knot nematode disease.关键词
烟草/根际/根结线虫/微生物群落/土壤酶活性/土壤化学性质Key words
tobacco/rhizosphere/root-knot nematodes/microbial community/soil enzyme activity/soil chemical properties分类
农业科技引用本文复制引用
袁国议,代惠娟,白羽祥,吴豪,李恩星,王戈,王娜,周鹏,杜宇,朱宏强,周艳宾..烟草健康株与根结线虫发病株根际微生态差异分析[J].中国烟草科学,2025,46(2):26-34,9.基金项目
云南省农业基础联合专项(202301BD070001-054) （202301BD070001-054）
国家自然科学基金项目(32160302、32260541、32160517) （32160302、32260541、32160517）
河北中烟工业有限责任公司科技项目(2022130000340019) （2022130000340019）
