中国烟草科学2025，Vol.46Issue(2)：35-44,10.DOI:10.13496/j.issn.1007-5119.2025.02.005
烟草靶斑病拮抗菌Y18鉴定及发酵条件优化
Identification of Tobacco Target Spot Antagonistic Strain Y18 and Optimisation of Its Fermentation Conditions
摘要
Abstract
Tobacco target spot disease,caused by Rhizoctonia solani,is one of the most important foliar diseases of tobacco.The screening and identification of biocontrol strains,along with the establishment of biocontrol fermentation conditions,can technically support the prevention and control of the disease,and the application of biocontrol bacteria.In this study,a previously screened antagonist strain Y18 was identified through the combination of morphology,physiology,biochemistry and molecular biology.Using the inhibition rate of Y18 on the mycelial growth of target tobacco blotch as an index,the fermentation medium components and fermentation conditions with the optimal inhibitory effect of Y18 on the target blotch were screened by one-way test and orthogonal test.The strain Y18 was identified as Bacillus velezensis,with the inhibition rate of 81.63%on Rhizoctonia solani.It also had pronounced inhibition on Phytophthora capsici,Fusarium graminearum,Phytophthora parasitica var.nicotianae,Ralstonia solanacearum and Pseudomonas syringae pv.tabaci,respectively,with the inhibitory rate reaching 79.08%,and the size of the inhibition circle for Ralstonia solanacearum up to 27.28 mm.The optimal fermentation medium,carbon source,nitrogen source and inorganic salt were NB medium,sucrose,beef paste and magnesium sulphate,respectively.The optimal medium components were 10 g sucrose,10 g beef paste and 7.5 g magnesium sulphate.The optimal fermentation conditions were pH=6,fermentation time of 48 h,inoculum amount of 5%and fermentation temperature of 26℃.The results can provide a theoretical basis for the development and utilization of strain Y18 as a biocontrol agent.关键词
烟草靶斑病/贝莱斯芽孢杆菌/拮抗作用/发酵优化Key words
tobacco target spot/Bacillus velezensis/antagonistic effect/fermentation optimization分类
植物保护学引用本文复制引用
肖越,刘天波,唐前君,熊有明,肖志鹏,刘峰,母婷婷,彭静,杨未,李玲玲,梅斯钇..烟草靶斑病拮抗菌Y18鉴定及发酵条件优化[J].中国烟草科学,2025,46(2):35-44,10.基金项目
中国烟草总公司湖南省公司项目[110202201027(LS-11)] （LS-11）
湖南省科学技术厅项目(2023NK2019) （2023NK2019）
湖南省烟草公司衡阳市公司项目(HYYC2023KJ32) （HYYC2023KJ32）
湖南省烟草公司永州市公司科技项目(YZ2023KJ02) （YZ2023KJ02）
湖南省农业科技创新资金项目(2023CX40) （2023CX40）
